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AB175200

Anti-OTUB1 antibody [EPR13028(B)]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

5

(3 Reviews)

|

(18 Publications)

Rabbit Recombinant Monoclonal OTUB1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

View Alternative Names

OTB1, OTU1, HSPC263, OTUB1, Ubiquitin thioesterase OTUB1, Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, hOTU1

6 Images
Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • IP

Lab

Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

ab175200 (purified) at 1 : 50 dilution (2ug) immunoprecipitating OTUB1 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab175200 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab175200 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (ab175200)

Predicted band size: 31 kDa

false

Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • IP

Supplier Data

Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate labeling OTUB1 with unpurified ab175200 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-OTUB1 antibody [EPR13028(B)] (ab175200)

Predicted band size: 31 kDa

false

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • WB

Lab

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

Lanes 1 - 4 : Merged signal (red and green). Green - ab175200 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab175200 was shown to specifically react with OTUB1 in wild type cells as signal was lost in OTUB1 knockout cells. Wild-type and OTUB1 knockout samples were subjected to SDS-PAGE. ab175200 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (ab175200) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

OTUB1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

HEK-293 whole cell lysate at 20 µg

Predicted band size: 31 kDa

false

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • WB

Lab

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

Lanes 1-2 : Merged signal (red and green). Green - ab175200 observed at 31 kDa. Red - loading control ab8245 observed at 37 kDa.

ab175200 Anti-OTUB1 antibody [EPR13028(B)] was shown to specifically react with OTUB1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266551 (knockout cell lysate ab257569) was used. Wild-type and OTUB1 knockout samples were subjected to SDS-PAGE. ab175200 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°CC at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (ab175200) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

OTUB1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human OTUB1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-otub1-knockout-hek-293t-cell-line-ab266551'>ab266551</a>)

Predicted band size: 31 kDa

Observed band size: 130 kDa,31 kDa

false

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • WB

Supplier Data

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

All lanes:

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (ab175200) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 31 kDa

false

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)
  • WB

Lab

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (AB175200)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-OTUB1 antibody [EPR13028(B)] (ab175200) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse heart whole tissue lysates at 15 µg

Lane 3:

Rat heart whole tissue lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

  • Carrier free

    Anti-OTUB1 antibody [EPR13028(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13028(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OTUB1 protein also known as OTU domain-containing ubiquitin aldehyde-binding protein 1 plays an important role in the removal of ubiquitin from proteins. This process is important for regulating protein stability and function within the cell. OTUB1 has a mass of approximately 32 kDa and is expressed ubiquitously across various tissues indicating its essential nature in cellular functions.
Biological function summary

OTUB1 functions as a deubiquitinating enzyme that cleaves ubiquitin from substrates influencing various cellular processes. It acts independently or as part of larger protein complexes. By controlling protein degradation pathways OTUB1 impacts protein turnover and cellular homeostasis. Interactions with other proteins allow OTUB1 to exert influence on different signaling pathways and cellular stress responses.

Pathways

OTUB1 holds a significant position in the regulation of the ubiquitin-proteasome system and DNA damage repair pathway. It closely interacts with proteins such as UBE2N affecting the DNA damage response. Additionally OTUB1 regulates processes through modulating pathways like NF-kB by interacting with and stabilizing specific protein factors involved.

OTUB1’s function connects it to various pathological states like cancer and neurodegenerative diseases. Aberrant expression or mutations in OTUB1 can lead to disruptions in cell cycle regulation and apoptosis contributing to oncogenesis. In the context of neurodegenerative diseases OTUB1 is related to tau stabilization with proteins such as HSP90 playing a role in the same regulatory mechanisms highlighting its relevance in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation (PubMed : 12401499, PubMed : 12704427, PubMed : 14661020, PubMed : 23827681). Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen (PubMed : 14661020). Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy (PubMed : 14661020). Isoform 1 destabilizes RNF128, leading to prevent anergy (PubMed : 14661020). In contrast, isoform 2 stabilizes RNF128 and promotes anergy (PubMed : 14661020). Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128 (PubMed : 14661020). Deubiquitinates estrogen receptor alpha (ESR1) (PubMed : 19383985). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains (PubMed : 18954305, PubMed : 19211026, PubMed : 23827681). Not able to cleave di-ubiquitin (PubMed : 18954305, PubMed : 23827681). Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin (PubMed : 18954305, PubMed : 23827681).. Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites (PubMed : 20725033, PubMed : 22325355). Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks (PubMed : 20725033, PubMed : 22325355). Inhibition occurs by binding to free ubiquitin : free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1 (PubMed : 20725033, PubMed : 22325355). The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain (PubMed : 20725033, PubMed : 22325355). Acts as a regulator of mTORC1 and mTORC2 complexes (PubMed : 29382726, PubMed : 35927303). When phosphorylated at Tyr-26, acts as an activator of the mTORC1 complex by mediating deubiquitination of RPTOR via a non-catalytic process : acts by binding and inhibiting the activity of the ubiquitin-conjugating enzyme E2 (UBE2D1/UBCH5A, UBE2W/UBC16 and UBE2N/UBC13), thereby preventing ubiquitination of RPTOR (PubMed : 35927303). Can also act as an inhibitor of the mTORC1 and mTORC2 complexes in response to amino acids by mediating non-catalytic deubiquitination of DEPTOR (PubMed : 29382726).
See full target information OTUB1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:2 PubMed39773263

2025

VCP regulates early tau seed amplification via specific cofactors.

Applications

Unspecified application

Species

Unspecified reactive species

Sushobhna Batra,Jaime Vaquer-Alicea,Clarissa Valdez,Skyler P Taylor,Victor A Manon,Anthony R Vega,Omar M Kashmer,Sourav Kolay,Andrew Lemoff,Nigel J Cairns,Charles L White,Marc I Diamond

International journal of biological sciences 20:3784-3801 PubMed39113709

2024

Target deubiquitinase OTUB1 as a therapeatic strategy for BLCA via β-catenin/necroptosis signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihao Liao,Jiaming Liang,Youzhi Wang,An Li,Wenbo Liu,Boqiang Zhong,Keke Wang,Diansheng Zhou,Tao Guo,Jianing Guo,Xi Yu,Ning Jiang

ACS pharmacology & translational science 7:176-185 PubMed38230274

2024

Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyue Li,Xiaoting Zhu,Ziying Sun,Qi Wang,Shaojiang Song,Yujia Xu,Guisong He,Xinliang Mao

Molecular neurobiology 61:1726-1736 PubMed37775718

2023

Deubiquitylating Enzyme OTUB1 Facilitates Neuronal Survival After Intracerebral Hemorrhage Via Inhibiting NF-κB-triggered Apoptotic Cascades.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabing Shen,Xiaoli Xue,Huimin Yuan,Yan Song,Jinglei Wang,Ronghui Cui,Kaifu Ke

Signal transduction and targeted therapy 8:142 PubMed37024477

2023

OTUB1 promotes osteoblastic bone formation through stabilizing FGFR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhu,Yesheng Fu,Chun-Ping Cui,Yi Ding,Zhikang Deng,Chao Ning,Fan Hu,Chen Qiu,Biyue Yu,Xuemei Zhou,Guan Yang,Jiang Peng,Weiguo Zou,Cui Hua Liu,Lingqiang Zhang

Oncogene 42:83-98 PubMed36369321

2022

CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1.

Applications

Unspecified application

Species

Unspecified reactive species

Dongbao Li,Yuhong Wang,Chao Dong,Tao Chen,Anqi Dong,Jiayu Ren,Weikang Li,Gege Shu,Jiaoyang Yang,Wenhao Shen,Lei Qin,Lin Hu,Jin Zhou

iScience 25:105363 PubMed36339263

2022

Ovarian tumorB1-mediated heat shock transcription factor 1 deubiquitination is critical for glycolysis and development of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Ling,Jiayi Lu,Xiaoyun Wang,Lan Liu,Lu Liu,Yadi Wang,Yujun Sun,Chune Ren,Chao Lu,Zhenhai Yu

Cell death & disease 13:271 PubMed35347117

2022

Mesenchymal stem cells protect against ferroptosis via exosome-mediated stabilization of SLC7A11 in acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyan Lin,Wenyi Chen,Jiahang Zhou,Jiaqi Zhu,Qigu Yao,Bing Feng,Xudong Feng,Xiaowei Shi,Qiaoling Pan,Jiong Yu,Lanjuan Li,Hongcui Cao

Nature chemical biology 18:412-421 PubMed35210618

2022

Deubiquitinase-targeting chimeras for targeted protein stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Nathaniel J Henning,Lydia Boike,Jessica N Spradlin,Carl C Ward,Gang Liu,Erika Zhang,Bridget P Belcher,Scott M Brittain,Matthew J Hesse,Dustin Dovala,Lynn M McGregor,Rachel Valdez Misiolek,Lindsey W Plasschaert,David J Rowlands,Feng Wang,Andreas O Frank,Daniel Fuller,Abigail R Estes,Katelyn L Randal,Anoohya Panidapu,Jeffrey M McKenna,John A Tallarico,Markus Schirle,Daniel K Nomura

Journal of cellular and molecular medicine 25:10980-10989 PubMed34773364

2021

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Peiyi Xie,Qing Chao,Jiuang Mao,Yue Liu,Jiayu Fang,Jing Xie,Jing Zhen,Yongqi Ding,Bidong Fu,Yun Ke,Da Huang
View all publications
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Product promise

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