Anti-OTUD1 antibody [EPR30462-31]

• WB

Lab

Western blot - Anti-OTUD1 antibody [EPR30462-31] (AB326105)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (51 kDa) are likely to be degraded target fragments.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-OTUD1 antibody [EPR30462-31] (ab326105) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

KARPAS-299 transfected with siRNA specifically targeting OTUD1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 51 kDa,36 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-OTUD1 antibody [EPR30462-31] (AB326105)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : K-562, PANC-1

The bands beneath the target band (51 kDa) are likely to be degraded target fragments.

Lane 1 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 125 seconds; Lanes 2-4 : 180 seconds

All lanes:

Western blot - Anti-OTUD1 antibody [EPR30462-31] (ab326105) at 1/1000 dilution

Lane 1:

AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 48 µg

Lane 2:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 48 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 48 µg

Lane 4:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 51 kDa,36 kDa

true