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AB316951

Anti-OTUD3 antibody

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Rabbit Polyclonal OTUD3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OTUD3.

View Alternative Names

KIAA0459, OTUD3, OTU domain-containing protein 3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-OTUD3 Antibody (AB316951)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OTUD3 Antibody (AB316951)

Immunofluorescence staining of U251 (Human brain glioma cell line) cells with ab316951 at 1/50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488® conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western blot - Anti-OTUD3 Antibody (AB316951)
  • WB

Supplier Data

Western blot - Anti-OTUD3 Antibody (AB316951)

All lanes:

Western blot - Anti-OTUD3 Antibody (ab316951) at 1/1000 dilution

Lane 1:

U-251 (Human brain glioma cell line) whole cell lysate

Lane 2:

U-87 (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OTUD3.

Q5T2D3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTUD3 also known as OTU Domain-Containing Protein 3 is a deubiquitinating enzyme that belongs to the OTU family. It has a molecular mass of approximately 48 kDa. OTUD3 is involved in removing ubiquitin from specific protein substrates impacting their degradation activity or localization. This protein is widely expressed in various tissues including the liver kidney and brain suggesting its roles in multiple physiological processes.
Biological function summary

OTUD3 is essential in regulating protein homeostasis and cellular signaling. It is part of protein complexes that modulate ubiquitination pathways maintaining proper protein turnover and function. By deubiquitinating target proteins OTUD3 can influence processes like cell cycle progression apoptosis and DNA repair. The enzyme's regulation of these pathways highlights its role in maintaining cellular health.

Pathways

OTUD3 influences key signaling cascades such as the NF-kB and p53 pathways. In the NF-kB pathway OTUD3 interacts with proteins like TRAF3 modulating inflammation and immune responses. Similarly in the p53 pathway it can impact the stability and activity of p53 protein which is critical for cell cycle regulation and apoptosis. The relationship of OTUD3 with these pathways indicates its importance in cell signaling and stress responses.

OTUD3 is connected to cancer and neurodegenerative diseases. Altered expression or activity of OTUD3 can contribute to tumorigenesis with changes seen in cancers like breast and lung cancer. OTUD3 is also linked to neurodegenerative conditions through its interaction with proteins like Parkin an important player in Parkinson's disease. Understanding the roles of OTUD3 in these diseases can guide therapeutic strategies and target designs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains (PubMed : 23827681, PubMed : 32011234, PubMed : 35675826). Important regulator of energy metabolism (PubMed : 35675826). Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation (PubMed : 35675826). In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation (PubMed : 35675826). Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination (PubMed : 32011234).
See full target information OTUD3
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Product promise

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