AB106368

Anti-OTUD4/HIN-1 antibody

Name: Anti-OTUD4/HIN-1 antibody (ab106368) | Abcam
Brand: Abcam Limited
SKU: AB106368
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal OTUD4/HIN-1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD4.

View Alternative Names

HIN-1, KIAA1046, OTUD4, OTU domain-containing protein 4, HIV-1-induced protein HIN-1

3 Images

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Immunocytochemistry - Anti-OTUD4/HIN-1 antibody (AB106368)

ab106368, at 2.5 ug/ml, staining OTUD4/HIN-1 in Daudi cells by immunocytochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-OTUD4/HIN-1 antibody (AB106368)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OTUD4/HIN-1 antibody (AB106368)

Immunofluorescence of OTUD4/HIN-1 in Daudi cells using ab106368 at 20 ug/ml.

Supplier Data

Western blot - Anti-OTUD4/HIN-1 antibody (AB106368)

Lane 1:

Western blot - Anti-OTUD4/HIN-1 antibody (ab106368) at 0.25 µg/mL

Lane 2:

Western blot - Anti-OTUD4/HIN-1 antibody (ab106368) at 0.5 µg/mL

All lanes:

Daudi (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 123 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, ICC/IF

applications

Immunogen

Synthetic Peptide within Human OTUD4. The exact immunogen used to generate this antibody is proprietary information.

Q01804

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2.5 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTUD4/HIN-1 also known as ovarian tumor domain-containing protein 4 is known for its deubiquitinating enzyme activity. This protein shows a molecular mass of approximately 135 kDa. It is expressed in various tissues with significant levels observed in the brain and kidneys. OTUD4 plays a role in regulating protein degradation by removing ubiquitin impacting cellular processes such as protein turnover cell cycle regulation and signaling pathways.

Biological function summary

The OTUD4 protein significantly influences cellular homeostasis and stress response. It participates in the protein quality control system as part of a complex that regulates the ubiquitin-proteasome system. This protein ensures the proper folding and degradation of misfolded proteins impacting cellular health and function. OTUD4 forms interactions with other proteins highlighting its role in modulating cellular stress responses and apoptosis.

Pathways

OTUD4 has connections with both the ubiquitin-proteasome and DNA damage response pathways. It participates in the modulation of these pathways ensuring that damaged or misfolded proteins do not accumulate. OTUD4 directly interacts with proteins such as ubiquitin-specific proteases and E3 ligases influencing apoptosis and stress response. These interactions reveal its involvement in cellular decisions related to repair survival or apoptosis.

OTUD4 shows associations with neurodegenerative diseases and certain cancers. Dysregulation in its function can relate to protein aggregation in neurodegenerative conditions with its expression affecting pathways related to proteins like Parkin and UCH-L1. In cancers changes in OTUD4 expression can impact tumor growth and survival pointing towards its potential for therapeutic targeting and biomarker use in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed : 23827681, PubMed : 25944111, PubMed : 29395066). May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed : 29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed : 25944111).

See full target information OTUD4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0230924 PubMed40277358

2025

Ubiquitin-dependent proteasomal degradation of small hepatitis B virus surface antigen mediated by TRIM21 and antagonized by OTUD4.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiang Wu,Zhihan Chen,Zhengqian Zhang,Jing Xu,Hang Li,Mengxian Lin,Wenjie Xie,Yan Chen,Xinjian Lin,Xu Lin

iScience 26:108515 PubMed38089580

2023

Cyclophilin A facilitates influenza B virus replication by stabilizing viral proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Huizi Li,Wenhui Fan,Jie Min,Xiaoyuan Bai,Wenxian Yang,Heqiao Li,Yuna Zhao,Runshan Lin,Xiaojuan Jia,Wenjun Liu,Lei Sun

Cell insight 2:100100 PubMed37193092

2023

The deubiquitinase OTUD4 inhibits the expression of antimicrobial peptides in Paneth cells to support intestinal inflammation and bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Yu,Yu-Yao Guo,Tianzi Liuyu,Peng Wang,Zhi-Dong Zhang,Dandan Lin,Bo Zhong

PloS one 11:e0150364 PubMed26934049

2016

Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles.

Applications

IHC

Species

Unspecified reactive species

Mariona Esquerdo,Xavier Grau-Bové,Alejandro Garanto,Vasileios Toulis,Sílvia Garcia-Monclús,Erica Millo,Ma José López-Iniesta,Víctor Abad-Morales,Iñaki Ruiz-Trillo,Gemma Marfany

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OTUD4/HIN-1 antibody

Immunocytochemistry - Anti-OTUD4/HIN-1 antibody (AB106368)

Immunocytochemistry/ Immunofluorescence - Anti-OTUD4/HIN-1 antibody (AB106368)

Western blot - Anti-OTUD4/HIN-1 antibody (AB106368)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise