Rabbit Polyclonal OTUD4/HIN-1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD4.
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- Cell insight 2:1001002023The deubiquitinase OTUD4 inhibits the expression of antimicrobial peptides in Paneth cells to support intestinal inflammation and bacterial infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeying Yu,Yu-Yao Guo,Tianzi Liuyu,Peng Wang,Zhi-Dong Zhang,Dandan Lin,Bo ZhongPubMed 37193092
- PloS one 11:e01503642016Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles.Applications:IHCReactive species:Unspecified reactive speciesMariona Esquerdo et. al.PubMed 26934049
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human OTUD4. The exact immunogen used to generate this antibody is proprietary information. Database link Q01804
Reactivity data
Select an application| ICC | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Target data
Function
Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed:23827681, PubMed:25944111, PubMed:29395066). May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111).
Alternative names
HIN-1, KIAA1046, OTUD4, OTU domain-containing protein 4, HIV-1-induced protein HIN-1
Recommended products
Rabbit Polyclonal OTUD4/HIN-1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human OTUD4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human OTUD4. The exact immunogen used to generate this antibody is proprietary information. Database link Q01804
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTUD4/HIN-1 also known as ovarian tumor domain-containing protein 4 is known for its deubiquitinating enzyme activity. This protein shows a molecular mass of approximately 135 kDa. It is expressed in various tissues with significant levels observed in the brain and kidneys. OTUD4 plays a role in regulating protein degradation by removing ubiquitin impacting cellular processes such as protein turnover cell cycle regulation and signaling pathways.
- Biological function summary
The OTUD4 protein significantly influences cellular homeostasis and stress response. It participates in the protein quality control system as part of a complex that regulates the ubiquitin-proteasome system. This protein ensures the proper folding and degradation of misfolded proteins impacting cellular health and function. OTUD4 forms interactions with other proteins highlighting its role in modulating cellular stress responses and apoptosis.
- Pathways
OTUD4 has connections with both the ubiquitin-proteasome and DNA damage response pathways. It participates in the modulation of these pathways ensuring that damaged or misfolded proteins do not accumulate. OTUD4 directly interacts with proteins such as ubiquitin-specific proteases and E3 ligases influencing apoptosis and stress response. These interactions reveal its involvement in cellular decisions related to repair survival or apoptosis.
- Associated diseases and disorders
OTUD4 shows associations with neurodegenerative diseases and certain cancers. Dysregulation in its function can relate to protein aggregation in neurodegenerative conditions with its expression affecting pathways related to proteins like Parkin and UCH-L1. In cancers changes in OTUD4 expression can impact tumor growth and survival pointing towards its potential for therapeutic targeting and biomarker use in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-OTUD4/HIN-1 antibody (ab106368)
Lane 1: Western blot - Anti-OTUD4/HIN-1 antibody (ab106368) at 0.25 µg/mL
Lane 2: Western blot - Anti-OTUD4/HIN-1 antibody (ab106368) at 0.5 µg/mL
All lanes: Daudi (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 123 kDa
Immunocytochemistry - Anti-OTUD4/HIN-1 antibody (ab106368)
ab106368, at 2.5 ug/ml, staining OTUD4/HIN-1 in Daudi cells by immunocytochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-OTUD4/HIN-1 antibody (ab106368)
Immunofluorescence of OTUD4/HIN-1 in Daudi cells using ab106368 at 20 ug/ml.
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