Rabbit Polyclonal OTUD6B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTUD6B aa 50 to C-terminus.
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- Frontiers in cellular and infection microbiology 13:10702712023USP8 inhibition regulates autophagy flux and controls infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Santelices,Mark Ou,Gustavo H B Maegawa,Kamil Hercik,Mariola J EdelmannPubMed 37026055
- The EMBO journal 41:e1108712022The OTUD6B-LIN28B-MYC axis determines the proliferative state in multiple myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Paulmann,Ria Spallek,Oleksandra Karpiuk,Michael Heider,Isabell Schäffer,Jana Zecha,Susan Klaeger,Michaela Walzik,Rupert Öllinger,Thomas Engleitner,Matthias Wirth,Ulrich Keller,Jan Krönke,Martina Rudelius,Susanne Kossatz,Roland Rad,Bernhard Kuster,Florian BassermannPubMed 36059274
- Frontiers in immunology 10:11002019OTUD6B-AS1 Might Be a Novel Regulator of Apoptosis in Systemic Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiki Takata et. al.PubMed 31156645
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OTUD6B aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N6M0
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Function
Isoform 1. Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (PubMed:21267069, PubMed:27864334). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (PubMed:27864334). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (PubMed:27864334). May also play a role in proteasome assembly and function (PubMed:28343629). Isoform 2. Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.
Alternative names
DUBA5, CGI-77, OTUD6B, Deubiquitinase OTUD6B, DUBA-5, OTU domain-containing protein 6B
Recommended products
Rabbit Polyclonal OTUD6B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTUD6B aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OTUD6B aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N6M0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTUD6B also known as OTU domain-containing protein 6B functions as a deubiquitinating enzyme. It specifically targets ubiquitin chains on substrate proteins for removal which regulates protein stability and activity. The molecular mass of OTUD6B is approximately 37 kDa. Researchers found its expression in a variety of tissues with higher levels in lung and placenta tissues. This suggests its importance in those areas but it also appears present in several other tissues throughout the body.
- Biological function summary
OTUD6B plays a significant role in cellular homeostasis by modulating protein turnover. Its enzymatic activity affects key regulatory proteins influencing cell cycle and signaling pathways. It does not seem to act within a larger protein complex but interacts individually with specific targets to exert its effects. Its deubiquitinating function impacts various cellular activities ensuring proper protein function and cell response.
- Pathways
OTUD6B integrates into ubiquitin-proteasome pathways and impacts the regulation of protein degradation and turnover. It closely associates with cellular processes like cell cycle progression and apoptosis. OTUD6B interacts with proteins such as p53 and NF-kB playing a part in their stabilization and activity regulation. These interactions show its role in important signaling pathways that influence cell fate and stress response.
- Associated diseases and disorders
Alterations in OTUD6B function connect to cancer and neurodegenerative diseases. Dysregulation of its deubiquitinating activity can contribute to tumorigenesis through inappropriate protein stabilization affecting tumor suppressors or oncogenes. In neurodegenerative conditions abnormalities in protein degradation pathways can arise where OTUD6B's function becomes critical. It is connected with proteins like p53 and tau which are implicated in cancer progression and Alzheimer's disease respectively. Understanding OTUD6B's role could offer insight into new therapeutic approaches for these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-OTUD6B antibody (ab127714)
12% SDS PAGE
All lanes: Western blot - Anti-OTUD6B antibody (ab127714) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-OTUD6B antibody (ab127714)
ab127714, at 1/500 dilution, staining OTUD6B in Paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is co-stained with Hoechst 33342.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-OTUD6B antibody (ab127714)
OTUD6B western blot using anti-OTUD6B antibody ab127714. Publication image and figure legend from Takata, M., Pachera, E., et al., 2019, Front Immunol, PubMed 31156645.
ab127714 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab127714 please see the product overview.
OTUD6B expression after OTUD6B-AS1 knockdown in dermal fibroblasts (Fb, A) or human pulmonary artery smooth muscle cells (HPASMC, B). HC dermal Fb or HPASMC were transfected with locked nucleic acid antisense oligonucleotide (ASO) negative control or with ASO targeting OTUD6B-AS1. (A) mRNA of total OTUD6B was measured 72 h after transfection (n = 5). OTUD6B protein was measured 72 h after transfection (n = 6) in dermal Fb by Western blot. (B) mRNA of total OTUD6B was measured 48 h after transfection (n = 4, biological replicates). OTUD6B protein expression was measured 48 h after transfection by Western blot (n = 4, biological replicates) in HPASMC. mRNA expression was normalized by GAPDH and RPLP0 and protein expression was normalized by GAPDH. (C) The subcellular localization of OTUD6B-AS1 in SSc dermal Fb was analyzed by qPCR (n = 4). Expression levels were normalized by GAPDH and RPLP0. Data are shown as single values and mean. Statistical analysis was performed by paired t-test. k.d, knockdown.
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