Anti-OTUD6B antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OTUD6B antibody (AB127714)

ab127714, at 1/500 dilution, staining OTUD6B in Paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is co-stained with Hoechst 33342.

• WB

Unknown

Western blot - Anti-OTUD6B antibody (AB127714)

12% SDS PAGE

All lanes:

Western blot - Anti-OTUD6B antibody (ab127714) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

• WB

CiteAb

Western blot - Anti-OTUD6B antibody (AB127714)

OTUD6B western blot using anti-OTUD6B antibody ab127714. Publication image and figure legend from Takata, M., Pachera, E., et al., 2019, Front Immunol, PubMed 31156645.

ab127714 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab127714 please see the product overview.

OTUD6B expression after OTUD6B-AS1 knockdown in dermal fibroblasts (Fb, A) or human pulmonary artery smooth muscle cells (HPASMC, B). HC dermal Fb or HPASMC were transfected with locked nucleic acid antisense oligonucleotide (ASO) negative control or with ASO targeting OTUD6B-AS1. (A) mRNA of total OTUD6B was measured 72 h after transfection (n = 5). OTUD6B protein was measured 72 h after transfection (n = 6) in dermal Fb by Western blot. (B) mRNA of total OTUD6B was measured 48 h after transfection (n = 4, biological replicates). OTUD6B protein expression was measured 48 h after transfection by Western blot (n = 4, biological replicates) in HPASMC. mRNA expression was normalized by GAPDH and RPLP0 and protein expression was normalized by GAPDH. (C) The subcellular localization of OTUD6B-AS1 in SSc dermal Fb was analyzed by qPCR (n = 4). Expression levels were normalized by GAPDH and RPLP0. Data are shown as single values and mean. Statistical analysis was performed by paired t-test. k.d, knockdown.

false