AB235326

Anti-OTUD6B antibody

Name: Anti-OTUD6B antibody (ab235326) | Abcam
Brand: Abcam Limited
SKU: AB235326

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(1 Publication)

Rabbit Polyclonal OTUD6B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OTUD6B.

View Alternative Names

DUBA5, CGI-77, OTUD6B, Deubiquitinase OTUD6B, DUBA-5, OTU domain-containing protein 6B

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD6B antibody (AB235326)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD6B antibody (AB235326)

Paraffin-embedded human ovarian cancer tissue stained for OTUD6B using ab235326 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD6B antibody (AB235326)

Paraffin-embedded human small intestine tissue stained for OTUD6B using ab235326 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-OTUD6B antibody (AB235326)

All lanes:

Western blot - Anti-OTUD6B antibody (ab235326) at 4 µg/mL

Lane 1:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human OTUD6B.

Q8N6M0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTUD6B also known as OTU domain-containing protein 6B functions as a deubiquitinating enzyme. It specifically targets ubiquitin chains on substrate proteins for removal which regulates protein stability and activity. The molecular mass of OTUD6B is approximately 37 kDa. Researchers found its expression in a variety of tissues with higher levels in lung and placenta tissues. This suggests its importance in those areas but it also appears present in several other tissues throughout the body.

Biological function summary

OTUD6B plays a significant role in cellular homeostasis by modulating protein turnover. Its enzymatic activity affects key regulatory proteins influencing cell cycle and signaling pathways. It does not seem to act within a larger protein complex but interacts individually with specific targets to exert its effects. Its deubiquitinating function impacts various cellular activities ensuring proper protein function and cell response.

Pathways

OTUD6B integrates into ubiquitin-proteasome pathways and impacts the regulation of protein degradation and turnover. It closely associates with cellular processes like cell cycle progression and apoptosis. OTUD6B interacts with proteins such as p53 and NF-kB playing a part in their stabilization and activity regulation. These interactions show its role in important signaling pathways that influence cell fate and stress response.

Alterations in OTUD6B function connect to cancer and neurodegenerative diseases. Dysregulation of its deubiquitinating activity can contribute to tumorigenesis through inappropriate protein stabilization affecting tumor suppressors or oncogenes. In neurodegenerative conditions abnormalities in protein degradation pathways can arise where OTUD6B's function becomes critical. It is connected with proteins like p53 and tau which are implicated in cancer progression and Alzheimer's disease respectively. Understanding OTUD6B's role could offer insight into new therapeutic approaches for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Isoform 1. Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (PubMed : 21267069, PubMed : 27864334). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (PubMed : 27864334). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (PubMed : 27864334). May also play a role in proteasome assembly and function (PubMed : 28343629).. Isoform 2. Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.

See full target information OTUD6B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107446 PubMed37599822

2023

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxiao Tong,Jia Rao,Chenxi Yang,Jie Xu,Yijun Lu,Yuchen Zhang,Xiaohui Cang,Shanshan Xie,Jianhua Mao,Pingping Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OTUD6B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD6B antibody (AB235326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTUD6B antibody (AB235326)

Western blot - Anti-OTUD6B antibody (AB235326)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise