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AB151117

Anti-OTULIN antibody

5

(3 Reviews)

|

(12 Publications)

Rabbit Polyclonal OTULIN antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTULIN aa 50-200.

View Alternative Names

FAM105B, OTULIN, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTULIN antibody (AB151117)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTULIN antibody (AB151117)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FAM105B with ab151117 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-OTULIN antibody (AB151117)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OTULIN antibody (AB151117)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and mitochondria. Recommended concentration of ab151117 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-OTULIN antibody (AB151117)
  • WB

Unknown

Western blot - Anti-OTULIN antibody (AB151117)

All lanes:

Western blot - Anti-OTULIN antibody (ab151117) at 1/100 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human OTULIN aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q96BN8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
Biological function summary

OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.

Pathways

OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.

OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed : 23708998, PubMed : 23746843, PubMed : 23806334, PubMed : 23827681, PubMed : 24726323, PubMed : 24726327, PubMed : 26997266, PubMed : 27523608, PubMed : 27559085, PubMed : 28919039, PubMed : 30804083, PubMed : 35170849, PubMed : 35587511, PubMed : 38630025, PubMed : 38652464). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed : 23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed : 23746843, PubMed : 23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex : acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed : 26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed : 27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed : 27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed : 23806334).
See full target information OTULIN
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:330 PubMed39721999

2024

OTULIN confers cisplatin resistance in osteosarcoma by mediating GPX4 protein homeostasis to evade the mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zehang Zheng,Yunhao Zeng,Xing Bao,Chuang Huang,Fengjing Guo,Fei Xu,Zhengqiang Luo

Nature communications 15:5515 PubMed38951495

2024

Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luan,Wenying Long,Lisi Dai,Panfeng Tao,Zhifen Deng,Zongping Xia

EMBO molecular medicine 14:e14901 PubMed35170849

2022

Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Zinngrebe,Barbara Moepps,Thomas Monecke,Peter Gierschik,Ferdinand Schlichtig,Thomas F E Barth,Gudrun Strauß,Elena Boldrin,Carsten Posovszky,Ansgar Schulz,Ortraud Beringer,Eva Rieser,Eva-Maria Jacobsen,Myriam Ricarda Lorenz,Klaus Schwarz,Ulrich Pannicke,Henning Walczak,Dierk Niessing,Catharina Schuetz,Pamela Fischer-Posovszky,Klaus-Michael Debatin

Cell reports 37:109777 PubMed34610306

2021

Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains.

Applications

Unspecified application

Species

Unspecified reactive species

Paul R Elliott,Derek Leske,Jane Wagstaff,Lisa Schlicher,Georgina Berridge,Sarah Maslen,Frederik Timmermann,Biao Ma,Roman Fischer,Stefan M V Freund,David Komander,Mads Gyrd-Hansen

Nature communications 12:5416 PubMed34518544

2021

Hypoxia regulates overall mRNA homeostasis by inducing Met-linked linear ubiquitination of AGO2 in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Zhang,Xian Zhao,Yanmin Guo,Ran Chen,Jianfeng He,Lian Li,Zhe Qiang,Qianqian Yang,Xiaojia Liu,Caihu Huang,Runhui Lu,Jiayu Fang,Yingting Cao,Jiayi Huang,Yanli Wang,Jian Huang,Guo-Qiang Chen,Jinke Cheng,Jianxiu Yu

BMC oral health 21:290 PubMed34092220

2021

Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Wei Fu,Hong-Zhi Xu

The EMBO journal 39:e103303 PubMed33215740

2020

Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lilian M Fennell,Carlos Gomez Diaz,Luiza Deszcz,Anoop Kavirayani,David Hoffmann,Kota Yanagitani,Alexander Schleiffer,Karl Mechtler,Astrid Hagelkruys,Josef Penninger,Fumiyo Ikeda

Cell reports 29:3652-3663.e5 PubMed31825842

2019

Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Todd Douglas,Maya Saleh

The EMBO journal 36:1147-1166 PubMed28258062

2017

The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.

Applications

WB

Species

Human

Elodie Lafont,Chahrazade Kantari-Mimoun,Peter Draber,Diego De Miguel,Torsten Hartwig,Matthias Reichert,Sebastian Kupka,Yutaka Shimizu,Lucia Taraborrelli,Maureen Spit,Martin R Sprick,Henning Walczak

Cell reports 16:2271-80 PubMed27545878

2016

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.

Applications

WB

Species

Unspecified reactive species

Sebastian Kupka,Diego De Miguel,Peter Draber,Luigi Martino,Silvia Surinova,Katrin Rittinger,Henning Walczak
View all publications
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Product promise

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