Rabbit Polyclonal OTULIN antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTULIN aa 50-200.
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- EMBO molecular medicine 14:e149012022Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Zinngrebe,Barbara Moepps,Thomas Monecke,Peter Gierschik,Ferdinand Schlichtig,Thomas F E Barth,Gudrun Strauß,Elena Boldrin,Carsten Posovszky,Ansgar Schulz,Ortraud Beringer,Eva Rieser,Eva-Maria Jacobsen,Myriam Ricarda Lorenz,Klaus Schwarz,Ulrich Pannicke,Henning Walczak,Dierk Niessing,Catharina Schuetz,Pamela Fischer-Posovszky,Klaus-Michael DebatinPubMed 35170849
- Cell reports 37:1097772021Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul R Elliott,Derek Leske,Jane Wagstaff,Lisa Schlicher,Georgina Berridge,Sarah Maslen,Frederik Timmermann,Biao Ma,Roman Fischer,Stefan M V Freund,David Komander,Mads Gyrd-HansenPubMed 34610306
- Nature communications 12:54162021Hypoxia regulates overall mRNA homeostasis by inducing Met-linked linear ubiquitination of AGO2 in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailong Zhang,Xian Zhao,Yanmin Guo,Ran Chen,Jianfeng He,Lian Li,Zhe Qiang,Qianqian Yang,Xiaojia Liu,Caihu Huang,Runhui Lu,Jiayu Fang,Yingting Cao,Jiayi Huang,Yanli Wang,Jian Huang,Guo-Qiang Chen,Jinke Cheng,Jianxiu YuPubMed 34518544
- BMC oral health 21:2902021Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Wei Fu,Hong-Zhi XuPubMed 34092220
- The EMBO journal 39:e1033032020Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLilian M Fennell,Carlos Gomez Diaz,Luiza Deszcz,Anoop Kavirayani,David Hoffmann,Kota Yanagitani,Alexander Schleiffer,Karl Mechtler,Astrid Hagelkruys,Josef Penninger,Fumiyo IkedaPubMed 33215740
- Cell reports 29:3652-3663.e52019Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTodd Douglas,Maya SalehPubMed 31825842
- The EMBO journal 36:1147-11662017The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.
- Cell reports 16:2271-802016SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
- EMBO reports 17:1485-14972016SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Schlicher,Manuela Wissler,Florian Preiss,Prisca Brauns-Schubert,Celia Jakob,Veronica Dumit,Christoph Borner,Joern Dengjel,Ulrich MaurerPubMed 27458237
- Nature communications 6:87772015The paracaspase MALT1 cleaves HOIL1 reducing linear ubiquitination by LUBAC to dampen lymphocyte NF-κB signalling.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OTULIN aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BN8
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:24726323, PubMed:24726327, PubMed:26997266, PubMed:27523608, PubMed:27559085, PubMed:28919039, PubMed:35170849, PubMed:35587511). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed:23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed:23746843, PubMed:23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed:26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed:27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed:27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed:23806334).
Alternative names
FAM105B, OTULIN, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby
Recommended products
Rabbit Polyclonal OTULIN antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OTULIN aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OTULIN aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BN8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
- Biological function summary
OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.
- Pathways
OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.
- Associated diseases and disorders
OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-OTULIN antibody (ab151117)
All lanes: Western blot - Anti-OTULIN antibody (ab151117) at 1/100 dilution
Lane 1: RT 4 cell lysate
Lane 2: U 251 MG cell lysate
Lane 3: Human plasma lysate
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-OTULIN antibody (ab151117)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and mitochondria. Recommended concentration of ab151117 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTULIN antibody (ab151117)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FAM105B with ab151117 at 1/50 dilution.
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