JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245652

Anti-OTULIN antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal OTULIN antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OTULIN aa 50-150.

View Alternative Names

FAM105B, OTULIN, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby

2 Images
Immunoprecipitation - Anti-OTULIN antibody (AB245652)
  • IP

Supplier Data

Immunoprecipitation - Anti-OTULIN antibody (AB245652)

OTULIN was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab245652 used for IP at 40 μL per reaction. For WB 1/1000 dilution.

Lane 1 : ab245652 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-OTULIN antibody (ab245652)

Predicted band size: 40 kDa

false

Western blot - Anti-OTULIN antibody (AB245652)
  • WB

Supplier Data

Western blot - Anti-OTULIN antibody (AB245652)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-OTULIN antibody (ab245652) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 40 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human OTULIN aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q96BN8

style
left-column

Alternative Products

Primary Antibodies

AB151117

Anti-OTULIN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTULIN antibody (AB151117)

  • 5
  • 3
Primary Antibodies

AB211328

Anti-OTULIN antibody [EPR19841]

RabMAb|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (AB211328)

  • 0
  • 0
Primary Antibodies

AB271992

Anti-OTULIN antibody [EPR19841] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB46322

Anti-RNF31/HOIP antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RNF31/HOIP antibody (AB46322)

  • 4
  • 5
Primary Antibodies

AB321891

Anti-SHARPIN antibody [EPR29120-74]

20ul selling size|Recombinant|RabMAb

Western blot - Anti-SHARPIN antibody [EPR29120-74] (AB321891)

  • 0
  • 0
Primary Antibodies

AB192618

Anti-USP14/TGT antibody [EPR15943] - C-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP14/TGT antibody [EPR15943] - C-terminal (AB192618)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20-40 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245652 was affinity purified using an epitope specific to OTULIN immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
Biological function summary

OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.

Pathways

OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.

OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed : 23708998, PubMed : 23746843, PubMed : 23806334, PubMed : 23827681, PubMed : 24726323, PubMed : 24726327, PubMed : 26997266, PubMed : 27523608, PubMed : 27559085, PubMed : 28919039, PubMed : 30804083, PubMed : 35170849, PubMed : 35587511, PubMed : 38630025, PubMed : 38652464). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed : 23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed : 23746843, PubMed : 23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex : acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed : 26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed : 27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed : 27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed : 23806334).
See full target information OTULIN
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:3241-3255 PubMed39072172

2024

RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells stabilizing phosphatase and tensin homolog mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-Xiang Wang,Feng Liu,Yi Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com