Rabbit Polyclonal OTULIN antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Ubiquitin thioesterase otulin aa 50-150.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human Ubiquitin thioesterase otulin aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BN8
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 20-40 μl/mg lysate |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:24726323, PubMed:24726327, PubMed:26997266, PubMed:27523608, PubMed:27559085, PubMed:28919039, PubMed:35170849, PubMed:35587511). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed:23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed:23746843, PubMed:23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed:26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed:27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed:27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed:23806334).
Alternative names
FAM105B, OTULIN, FAM105B, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby
Recommended products
Rabbit Polyclonal OTULIN antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Ubiquitin thioesterase otulin aa 50-150.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human Ubiquitin thioesterase otulin aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BN8
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab245652 was affinity purified using an epitope specific to OTULIN immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
- Biological function summary
OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.
- Pathways
OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.
- Associated diseases and disorders
OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-OTULIN antibody (ab245652)
Prepared using NETN lysis buffer.
All lanes: Western blot - Anti-OTULIN antibody (ab245652) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 4: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 10s
Immunoprecipitation - Anti-OTULIN antibody (ab245652)
OTULIN was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245652 used for IP at 40 μL per reaction. For WB 1/1000 dilution.
Lane 1: ab245652 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 10 seconds.
All lanes: Immunoprecipitation - Anti-OTULIN antibody (ab245652)
Predicted band size: 40 kDa
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