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Rabbit Recombinant Monoclonal OTULIN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-OTULIN antibody [EPR19841] (AB211328), expandable thumbnail
  • Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (AB211328), expandable thumbnail
  • Western blot - Anti-OTULIN antibody [EPR19841] (AB211328), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed:26997266, PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:27523608, PubMed:27559085, PubMed:24726323, PubMed:24726327, PubMed:28919039). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed:23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed:23746843, PubMed:23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed:26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed:27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed:27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling (PubMed:23806334).

Alternative names

Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby, OTULIN, FAM105B

Recommended products

Rabbit Recombinant Monoclonal OTULIN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19841

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.

Biological function summary

OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.

Pathways

OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.

Associated diseases and disorders

OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-OTULIN antibody [EPR19841] (ab211328), expandable thumbnail

    Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

    Lane 2: OTULIN knockout HEK-293T whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

    Exposure time: 2s

  • Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328), expandable thumbnail

    Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)

    OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: HEK-293T whole cell lysate 10 μg (Input).

    Lane 2: ab211328 IP in HEK-293T whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211328 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-OTULIN antibody [EPR19841] (ab211328), expandable thumbnail

    Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)

    Exposure time : Lane 1-3: 3 minutes; Lane 4-5: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

    Lane 1: Human brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal spleen lysate at 10 µg

    Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 5: PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    Lanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 4 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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