AB211328

Anti-OTULIN antibody [EPR19841]

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal OTULIN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

FAM105B, OTULIN, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby

3 Images

Supplier Data

Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (AB211328)

OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : HEK-293T whole cell lysate 10 μg (Input).

Lane 2 : ab211328 IP in HEK-293T whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211328 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Supplier Data

Western blot - Anti-OTULIN antibody [EPR19841] (AB211328)

Exposure time : Lane 1-3 : 3 minutes; Lane 4-5 : 3 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal spleen lysate at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 5:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 4 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

true

Supplier Data

Western blot - Anti-OTULIN antibody [EPR19841] (AB211328)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

OTULIN knockout HEK-293T whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

true

Exposure time: 2s

Carrier free

Anti-OTULIN antibody [EPR19841] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19841

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.

Biological function summary

OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.

Pathways

OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.

OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed : 23708998, PubMed : 23746843, PubMed : 23806334, PubMed : 23827681, PubMed : 24726323, PubMed : 24726327, PubMed : 26997266, PubMed : 27523608, PubMed : 27559085, PubMed : 28919039, PubMed : 30804083, PubMed : 35170849, PubMed : 35587511, PubMed : 38630025, PubMed : 38652464). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed : 23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed : 23746843, PubMed : 23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex : acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed : 26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed : 27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed : 27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed : 23806334).

See full target information OTULIN

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:257-267 PubMed40963028

2025

Basal cell of origin resolves neuroendocrine-tuft lineage plasticity in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie S Ireland,Daniel A Xie,Sarah B Hawgood,Margaret W Barbier,Lisa Y Zuo,Benjamin E Hanna,Scarlett Lucas-Randolph,Darren R Tyson,Benjamin L Witt,Ramaswamy Govindan,Afshin Dowlati,Justin C Moser,Anish Thomas,Sonam Puri,Charles M Rudin,Joseph M Chan,Andrew Elliott,Trudy G Oliver

Journal of experimental & clinical cancer research : CR 43:330 PubMed39721999

2024

OTULIN confers cisplatin resistance in osteosarcoma by mediating GPX4 protein homeostasis to evade the mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zehang Zheng,Yunhao Zeng,Xing Bao,Chuang Huang,Fengjing Guo,Fei Xu,Zhengqiang Luo

The Journal of cell biology 223: PubMed38319288

2024

TNF and IFNγ-induced cell death requires IRF1 and ELAVL1 to promote CASP8 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Buhao Deng,Jingyi Wang,Tingyun Yang,Zhao Deng,Jiafan Yuan,Bohan Zhang,Zhen Zhou,Fang Chen,Lu Fang,Chengzhi Liang,Bo Yan,Youwei Ai

Oncogenesis 13:6 PubMed38272870

2024

LUBAC promotes angiogenesis and lung tumorigenesis by ubiquitinating and antagonizing autophagic degradation of HIF1α.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Jin,Yazhi Peng,Jie Xu,Ye Yuan,Nan Yang,Zemei Zhang,Lei Xu,Lin Li,Yulian Xiong,Dejiao Sun,Yamu Pan,Ruiqing Wu,Jian Fu

Cell & bioscience 12:154 PubMed36088389

2022

QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Zhai,Wenwen Wang,Zichen Ye,Ke Xue,Guo Chen,Sijun Hu,Zhao Yan,Yanhai Guo,Fang Wang,Xubo Li,An Xiang,Xia Li,Zifan Lu,Li Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OTULIN antibody [EPR19841]

Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (AB211328)

Western blot - Anti-OTULIN antibody [EPR19841] (AB211328)

Western blot - Anti-OTULIN antibody [EPR19841] (AB211328)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise