Rabbit Recombinant Monoclonal OTULIN antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--western-blot-img164404.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992)")
![Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--immunoprecipitation-img5917.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992)")
![Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--western-blot-img172969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (AB271992)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed:26997266, PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:27523608, PubMed:27559085, PubMed:24726323, PubMed:24726327, PubMed:28919039). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed:23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed:23746843, PubMed:23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed:26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed:27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed:27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling (PubMed:23806334).
Alternative names
Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby, OTULIN, FAM105B
Recommended products
Rabbit Recombinant Monoclonal OTULIN antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19841
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab271992 is the carrier-free version of Anti-OTULIN antibody [EPR19841] ab211328.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
- Biological function summary
OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.
- Pathways
OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.
- Associated diseases and disorders
OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--western-blot-img164404.jpg "Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)")
Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
This data was developed using Anti-OTULIN antibody [EPR19841] ab211328, the same antibody clone in a different buffer formulation
Exposure time : Lane 1-3: 3 minutes; Lane 4-5: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992) at 1/1000 dilution
Lane 1: Human brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal spleen lysate at 10 µg
Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 5: PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 4 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--immunoprecipitation-img5917.jpg "Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)")
Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with Anti-OTULIN antibody [EPR19841] ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-OTULIN antibody [EPR19841] ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: Anti-OTULIN antibody [EPR19841] ab211328 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-OTULIN antibody [EPR19841] ab211328 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-OTULIN antibody [EPR19841] ab211328).
All lanes: Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (Anti-OTULIN antibody [EPR19841] ab211328)
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992), expandable thumbnail](https://content.abcam.com/products/images/otulin-antibody-epr19841-bsa-and-azide-free-ab271992--western-blot-img172969.jpg "Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)")
Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
This data was developed using Anti-OTULIN antibody [EPR19841] ab211328, the same antibody clone in a different buffer formulation:
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 2: OTULIN knockout HEK-293T whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 2s
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