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Rabbit Recombinant Monoclonal OTX1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 3 publications.

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Images

Western blot - Anti-Otx1 antibody [EPR3347] (AB92515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (AB92515), expandable thumbnail
  • Western blot - Anti-Otx1 antibody [EPR3347] (AB92515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (AB92515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (AB92515), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/20

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/1000

Notes

We recommend 1/500 dilution for human and mouse tissues, and 1/1000 for rat tissues.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500 - 1/1000

Notes

We recommend 1/500 dilution for human and mouse tissues, and 1/1000 for rat tissues.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500 - 1/1000

Notes

We recommend 1/500 dilution for human and mouse tissues, and 1/1000 for rat tissues.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Alternative names

Homeobox protein OTX1, Orthodenticle homolog 1, OTX1

Recommended products

Rabbit Recombinant Monoclonal OTX1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3347

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Otx1 also known as Orthodenticle homeobox 1 is a transcription factor with a noted molecular weight of approximately 32 kDa. It identifies as a homeodomain-containing protein that binds to specific DNA sequences regulating gene expression. This protein finds expression mainly in the developing and adult brain where it plays significant roles in brain morphogenesis. In addition to the central nervous system Otx1 displays expression in the retina and skeletal tissues during embryonic development.

Biological function summary

Otx1 functions in the regulation of several critical developmental processes. It contains a homeodomain which allows the protein to precisely bind to DNA influencing the transcriptional activation of target genes. Although it does not participate in large protein complexes it exerts influence in morphogenesis by its interaction with co-factors that regulate gene expression important for brain and sensory organ development. Its expression is pivotal in maintaining the balance of excitatory and inhibitory signals during cortical development.

Pathways

Otx1 integrates into signaling networks essential for developmental processes. It participates actively in pathways like the Wnt signaling pathway and the Sonic Hedgehog (Shh) pathway both contributing significantly to brain development and patterning. Within these pathways it interacts closely with proteins like β-catenin in the Wnt pathway and Gli transcription factors in the Shh pathway steering the developmental fates of neural progenitors.

Associated diseases and disorders

Otx1 contributes to conditions affecting the central nervous system. Mutations or dysregulation of Otx1 have connections to intellectual disabilities and specific epilepsy forms due to its role in cortical neuron development and function. The protein's interactions with other developmental regulators such as Pax6 offer insights into congenital neural developmental disorders emphasizing its critical role alongside other homeodomain proteins in maintaining proper neural development and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Western blot - Anti-Otx1 antibody [EPR3347] (ab92515)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Otx1 antibody [EPR3347] (ab92515) at 1/2500 dilution

    All lanes: Y79 cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 37 kDa

    Observed band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human retina tissue labelling Otx1 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Nuclear staining on inner nuclear layer and outer nuclear layer of the human retina.

  • Western blot - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Western blot - Anti-Otx1 antibody [EPR3347] (ab92515)

    All lanes: Western blot - Anti-Otx1 antibody [EPR3347] (ab92515) at 1 µg/mL

    All lanes: OTX1 Recombinant Protein at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa

    Observed band size: 75 kDa

    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human retinoblastoma tissue labelling Otx1 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Nuclear staining on tumor cells of human retinoblastoma.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse retina tissue labelling Otx1 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Nuclear staining on nuclear layer of the mouse retina.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14.5 choroid plexus tissue labelling Otx1 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Nuclear staining on mouse E14.5 choroid plexus.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat E14.5 choroid plexus tissue labelling Otx1 with purified ab92515 at a dilution 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Nuclear staining on rat E14.5 choroid plexus.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody [EPR3347] (ab92515)

    Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab92515 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunoprecipitation - Anti-Otx1 antibody [EPR3347] (ab92515), expandable thumbnail

    Immunoprecipitation - Anti-Otx1 antibody [EPR3347] (ab92515)

    Otx1 was immunoprecipitated from Y79（Human retinoblastoma retinoblastoma）whole cell lysate with ab92515 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab92515 at 1/500 dilution. Secondary antibody details (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-Otx1 antibody [EPR3347] (ab92515) at 1/500 dilution

    Lane 1: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate at 10 µg

    Lanes 2 - 3: Y79 whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 37 kDa

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