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AB76748

Anti-Otx2 antibody

1

(1 Review)

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(2 Publications)

Rabbit Polyclonal OTX2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human OTX2.

View Alternative Names

Homeobox protein OTX2, Orthodenticle homolog 2, OTX2

1 Images
Western blot - Anti-Otx2 antibody (AB76748)
  • WB

Unknown

Western blot - Anti-Otx2 antibody (AB76748)

Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Otx2 antibody (ab76748) at 1 µg/mL

Lane 1:

OTX1 Recombinant Protein at 0.1 µg

Lane 2:

OTX2 Overexpression Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 32 kDa

Observed band size: 37 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Synthetic Peptide within Human OTX2. The exact immunogen used to generate this antibody is proprietary information.

P32243

Specificity

Reacts specifically with 31 kDa Otx2 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Otx2 also known as Orthodenticle homeobox 2 is a transcription factor involved in the regulation of gene expression. With a mass of approximately 34 kDa it expresses mainly in the brain and eyes during embryonic development. Otx2 plays a central role in early patterning of the head and is essential for the development of structures such as the brain and retina. During adulthood its expression continues although in a more restricted manner within specific regions of the brain.
Biological function summary

Otx2 regulates the expression of genes necessary for cell differentiation and organ development. It functions as part of transcriptional complexes that control specific patterns of development in the central nervous system. This protein helps in maintaining the regional identity of cells ensuring the proper formation and function of neural tissues. By influencing key developmental processes Otx2 shapes the intricate architecture of the brain and contributes to its complexity.

Pathways

Otx2 integrates into the Wnt signaling and Hedgehog pathways which are critical for early embryonic development. It interacts with proteins such as Pax6 and En1 within these pathways to modulate developmental signals and maintain proper cellular functions. Through precise regulation of these pathways Otx2 ensures coordinated developmental processes that establish proper tissue and organ formation.

Otx2 plays an important role in certain developmental eye disorders and brain structural defects. Mutations or malfunctions in Otx2 expression can lead to conditions such as anophthalmia or microphthalmia and pituitary gland abnormalities. Otx2's interplay with proteins like Pax6 is significant in these disorders as disruptions in their functions can result in profound developmental consequences highlighting the importance of Otx2 in ocular and neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS) : 5'-TCTAATCCC-3'.
See full target information OTX2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34545806

2021

Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tania Moreno-Mármol,Mario Ledesma-Terrón,Noemi Tabanera,Maria Jesús Martin-Bermejo,Marcos J Cardozo,Florencia Cavodeassi,Paola Bovolenta

Journal of neuropathology and experimental neurology 74:110-20 PubMed25575132

2015

High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Sophia Japp,Marco Gessi,Martina Messing-Jünger,Dorota Denkhaus,Anja Zur Mühlen,Johannes Ernst Wolff,Stefan Hartung,Uwe Kordes,Ludger Klein-Hitpass,Torsten Pietsch
View all publications
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