Rabbit Polyclonal OTX2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human OTX2.
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- eLife 10:2021Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTania Moreno-Mármol,Mario Ledesma-Terrón,Noemi Tabanera,Maria Jesús Martin-Bermejo,Marcos J Cardozo,Florencia Cavodeassi,Paola BovolentaPubMed 34545806
- Journal of neuropathology and experimental neurology 74:110-202015High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Sophia Japp,Marco Gessi,Martina Messing-Jünger,Dorota Denkhaus,Anja Zur Mühlen,Johannes Ernst Wolff,Stefan Hartung,Uwe Kordes,Ludger Klein-Hitpass,Torsten PietschPubMed 25575132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human OTX2. The exact immunogen used to generate this antibody is proprietary information. Database link P32243
Reactivity data
Select an application| WB | |
|---|---|
| Rat | Predicted |
| Recombinant full length protein - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Alternative names
Homeobox protein OTX2, Orthodenticle homolog 2, OTX2
Recommended products
Rabbit Polyclonal OTX2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human OTX2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human OTX2. The exact immunogen used to generate this antibody is proprietary information. Database link P32243
- Purity
- Whole antiserum
- Specificity
Reacts specifically with 31 kDa Otx2 protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Otx2 also known as Orthodenticle homeobox 2 is a transcription factor involved in the regulation of gene expression. With a mass of approximately 34 kDa it expresses mainly in the brain and eyes during embryonic development. Otx2 plays a central role in early patterning of the head and is essential for the development of structures such as the brain and retina. During adulthood its expression continues although in a more restricted manner within specific regions of the brain.
- Biological function summary
Otx2 regulates the expression of genes necessary for cell differentiation and organ development. It functions as part of transcriptional complexes that control specific patterns of development in the central nervous system. This protein helps in maintaining the regional identity of cells ensuring the proper formation and function of neural tissues. By influencing key developmental processes Otx2 shapes the intricate architecture of the brain and contributes to its complexity.
- Pathways
Otx2 integrates into the Wnt signaling and Hedgehog pathways which are critical for early embryonic development. It interacts with proteins such as Pax6 and En1 within these pathways to modulate developmental signals and maintain proper cellular functions. Through precise regulation of these pathways Otx2 ensures coordinated developmental processes that establish proper tissue and organ formation.
- Associated diseases and disorders
Otx2 plays an important role in certain developmental eye disorders and brain structural defects. Mutations or malfunctions in Otx2 expression can lead to conditions such as anophthalmia or microphthalmia and pituitary gland abnormalities. Otx2's interplay with proteins like Pax6 is significant in these disorders as disruptions in their functions can result in profound developmental consequences highlighting the importance of Otx2 in ocular and neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Otx2 antibody (ab76748)
Otx1 Recombinant Protein was used as a negative control.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.All lanes: Western blot - Anti-Otx2 antibody (ab76748) at 1 µg/mL
Lane 1: OTX1 Recombinant Protein at 0.1 µg
Lane 2: OTX2 Overexpression Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa
Exposure time: 8min
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