Name: Anti-Otx2 antibody [EPR20375]
SKU: ab183951
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal OTX2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Related conjugates and formulations

ab222495
Carrier free
Anti-Otx2 antibody [EPR20375] - BSA and Azide free
ab319378
Conjugated
PE Anti-Otx2 antibody [EPR20375]
ab319512
Conjugated
Alexa Fluor® 488 Anti-Otx2 antibody [EPR20375]
ab319230
Conjugated
APC Anti-Otx2 antibody [EPR20375]
ab319625
Conjugated
Alexa Fluor® 647 Anti-Otx2 antibody [EPR20375]
ab319729
Conjugated
Alexa Fluor® 594 Anti-Otx2 antibody [EPR20375]
ab319871
Conjugated
Alexa Fluor® 555 Anti-Otx2 antibody [EPR20375]
ab321285
Conjugated
Alexa Fluor® 750 Anti-Otx2 antibody [EPR20375]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (AB183951), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Tested	Tested	Tested
Mouse	Expected	Expected	Tested
Rat	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

See full target information OTX2

Function

Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Alternative names

Homeobox protein OTX2, Orthodenticle homolog 2, OTX2

Recommended products

Rabbit Recombinant Monoclonal OTX2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20375
Purification technique: Affinity purification Protein A
Specificity: This antibody recognizes isoform 1 and isoform 2 of Otx2.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Otx2 also known as Orthodenticle homeobox 2 is a transcription factor involved in the regulation of gene expression. With a mass of approximately 34 kDa it expresses mainly in the brain and eyes during embryonic development. Otx2 plays a central role in early patterning of the head and is essential for the development of structures such as the brain and retina. During adulthood its expression continues although in a more restricted manner within specific regions of the brain.

Biological function summary

Otx2 regulates the expression of genes necessary for cell differentiation and organ development. It functions as part of transcriptional complexes that control specific patterns of development in the central nervous system. This protein helps in maintaining the regional identity of cells ensuring the proper formation and function of neural tissues. By influencing key developmental processes Otx2 shapes the intricate architecture of the brain and contributes to its complexity.

Pathways

Otx2 integrates into the Wnt signaling and Hedgehog pathways which are critical for early embryonic development. It interacts with proteins such as Pax6 and En1 within these pathways to modulate developmental signals and maintain proper cellular functions. Through precise regulation of these pathways Otx2 ensures coordinated developmental processes that establish proper tissue and organ formation.

Associated diseases and disorders

Otx2 plays an important role in certain developmental eye disorders and brain structural defects. Mutations or malfunctions in Otx2 expression can lead to conditions such as anophthalmia or microphthalmia and pituitary gland abnormalities. Otx2's interplay with proteins like Pax6 is significant in these disorders as disruptions in their functions can result in profound developmental consequences highlighting the importance of Otx2 in ocular and neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
IHC image of Otx2 staining in a section of formalin-fixed paraffin-embedded normal mouse e14 foetal brain performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab183951, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
IHC image of Otx2 staining in a section of formalin-fixed paraffin-embedded normal mouse e14 foetus performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab183951, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
Immunohistochemical analysis of paraffin-embedded rat E14.5 choroid plexus tissue labeling Otx2 with ab183951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on choroid plexus of E14.5 rat is observed [PMID: 23364326]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
Immunohistochemical analysis of paraffin-embedded human retina tissue labeling Otx2 with ab183951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on inner nuclear layer and outer nuclear layer of the human retina is observed [PMID: 19686387]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue labeling Otx2 with ab183951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on tumor cells of human retinoblastoma is observed [PMID: 19686387]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 choroid plexus tissue labeling Otx2 with ab183951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on choroid plexus of E14.5 mouse is observed [PMID: 23364326]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Otx2 antibody [EPR20375] (ab183951)
Otx2 was immunoprecipitated from 0.35 mg of Y79 (Human retinoblastoma cell line) whole cell lysate with ab183951 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Y79 whole cell lysate, 10 μg (Input).
Lane 2: ab183951 IP in Y79 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183951 in Y79 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-Otx2 antibody [EPR20375] (ab183951)
Predicted band size: 32 kDa
Observed band size: 32 kDa, 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx2 antibody [EPR20375] (ab183951)
Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling Otx2 with ab183951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on nuclear layer of the mouse retina is observed [PMID: 19686387]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Otx2 antibody [EPR20375] (ab183951)
Blocking/Dilution buffer: 5% NFDM/TBST.
Negative control: HeLa (PMID: 12559959).
This antibody recognizes isoform 1 and isoform 2 of Otx2.
All lanes: Western blot - Anti-Otx2 antibody [EPR20375] (ab183951) at 1/5000 dilution
Lane 1: Y79 (Human retinoblastoma cell line) whole cell lysate at 20 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 32 kDa, 49 kDa
Observed band size: 32 kDa, 34 kDa, 41 kDa
Exposure time: 5s