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Rabbit Recombinant Monoclonal OTX2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (AB247542), expandable thumbnail
  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (AB247542), expandable thumbnail
  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (AB247542), expandable thumbnail
  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (AB247542), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (AB247542), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Tested
Not recommended
Mouse
Tested
Not recommended
Expected
Expected
Not recommended
Rat
Tested
Not recommended
Expected
Expected
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Alternative names

Homeobox protein OTX2, Orthodenticle homolog 2, OTX2

Recommended products

Rabbit Recombinant Monoclonal OTX2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3348
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247542 is the carrier-free version of Anti-Otx2 antibody [EPR3348] ab92326.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Otx2 also known as Orthodenticle homeobox 2 is a transcription factor involved in the regulation of gene expression. With a mass of approximately 34 kDa it expresses mainly in the brain and eyes during embryonic development. Otx2 plays a central role in early patterning of the head and is essential for the development of structures such as the brain and retina. During adulthood its expression continues although in a more restricted manner within specific regions of the brain.

Biological function summary

Otx2 regulates the expression of genes necessary for cell differentiation and organ development. It functions as part of transcriptional complexes that control specific patterns of development in the central nervous system. This protein helps in maintaining the regional identity of cells ensuring the proper formation and function of neural tissues. By influencing key developmental processes Otx2 shapes the intricate architecture of the brain and contributes to its complexity.

Pathways

Otx2 integrates into the Wnt signaling and Hedgehog pathways which are critical for early embryonic development. It interacts with proteins such as Pax6 and En1 within these pathways to modulate developmental signals and maintain proper cellular functions. Through precise regulation of these pathways Otx2 ensures coordinated developmental processes that establish proper tissue and organ formation.

Associated diseases and disorders

Otx2 plays an important role in certain developmental eye disorders and brain structural defects. Mutations or malfunctions in Otx2 expression can lead to conditions such as anophthalmia or microphthalmia and pituitary gland abnormalities. Otx2's interplay with proteins like Pax6 is significant in these disorders as disruptions in their functions can result in profound developmental consequences highlighting the importance of Otx2 in ocular and neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    This data was developed using Anti-Otx2 antibody [EPR3348] ab92326, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Otx2 antibody [EPR3348] (Anti-Otx2 antibody [EPR3348] ab92326) at 1/1000 dilution

    All lanes: Y79 cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    This data was developed using Anti-Otx2 antibody [EPR3348] ab92326, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Otx2 antibody [EPR3348] (Anti-Otx2 antibody [EPR3348] ab92326) at 1/1000 dilution

    All lanes: Y79 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    Otx2 Western blot staining using rabbit Anti-Otx2 antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Otx2 antibody [EPR3348] ab92326).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 was used as a Vinculin loading control at a 1/10000 dilution.

    Negative control: spleen
    The membrane was incubated with Anti-Otx2 antibody [EPR3348] ab92326 at 4° overnight.

    All lanes: Western blot - Anti-Otx2 antibody [EPR3348] (Anti-Otx2 antibody [EPR3348] ab92326) at 1/1000 dilution

    Lane 1: Mouse retina tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 36 kDa

    Exposure time: 70s

  • Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Western blot - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    Otx2 Western blot staining using rabbit Anti-Otx2 antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Otx2 antibody [EPR3348] ab92326).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 was used as a Vinculin loading control at a 1/10000 dilution.

    Negative control: spleen

    All lanes: Western blot - Anti-Otx2 antibody [EPR3348] (Anti-Otx2 antibody [EPR3348] ab92326) at 1/1000 dilution

    Lane 1: Rat retina tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 32 kDa

    Exposure time: 147s

  • Flow Cytometry (Intracellular) - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    This data was developed using Anti-Otx2 antibody [EPR3348] ab92326, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20l permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / Y79(human retinoblastoma cell, Right) cells labelling Otx2 with Anti-Otx2 antibody [EPR3348] ab92326 at 1/1000 dilution (0.01µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 12559959).

  • Immunocytochemistry/ Immunofluorescence - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Otx2 antibody [EPR3348] - BSA and Azide free (ab247542)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Otx2 antibody [EPR3348] ab92326).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Y79 (human retinoblastoma cell) cells labelling Otx2 with Anti-Otx2 antibody [EPR3348] ab92326 at 1/500 (2.098 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

    Confocal image showing nuclear staining in Y79 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
    Negative control: HeLa (PMID: 12559959).
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Magenta).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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