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AB181688

Anti-Ovalbumin antibody

3

(3 Reviews)

|

(15 Publications)

Anti-Ovalbumin antibody (ab181688) is a rabbit polyclonal antibody detecting Ovalbumin in Western Blot, IP, IHC-P, ELISA. Suitable for Chicken.

View Alternative Names

Ovalbumin, Allergen Gal d II, Egg albumin, Plakalbumin, SERPINB14, OVA

3 Images
Western blot - Anti-Ovalbumin antibody (AB181688)
  • WB

Supplier Data

Western blot - Anti-Ovalbumin antibody (AB181688)

Lane 1 + 2 under reducing conditions; Lane 3 + 4 under non-reducing conditions.

All lanes:

Western blot - Anti-Ovalbumin antibody (ab181688) at 1/5000 dilution

Lanes 1 and 3:

Ovalbumin protein at 1 µg

Lanes 2 and 4:

Ovalbumin protein at 0.25 µg

Secondary

All lanes:

Atto 425 conjugated goat anti rabbit secondary antibody at 1/10000 dilution

Observed band size: 36 kDa

false

Western blot - Anti-Ovalbumin antibody (AB181688)
  • WB

Supplier Data

Western blot - Anti-Ovalbumin antibody (AB181688)

All lanes:

Western blot - Anti-Ovalbumin antibody (ab181688) at 1/1000 dilution

All lanes:

Reduced Ovalbumin at 0.005 µg

Secondary

All lanes:

Peroxidase rabbit secondary antibody 30 min at RT at 1/40000 dilution

Observed band size: 45 kDa

false

Immunohistochemistry - Anti-Ovalbumin antibody (AB181688)
  • IHC

CiteAb

Immunohistochemistry - Anti-Ovalbumin antibody (AB181688)

Ovalbumin immunohistochemistry using Anti-Ovalbumin antibody ab181688. Publication image and figure legend from Zeng, Z., Gao, J., et al., 2023, Int J Biol Sci, Pubmed 37416767.

NADK promotes the lymph node metastasis of NSCLC cells. Figure 3A. The schematic illustration of LN metastasis model preformed on C57BL/6 mice. 3B. LN metastasis model was conducted on C57BL/6 mice with LLC-NADK overexpression cells, popliteal lymph nodes were isolated at the end of the experiment, and LN volume was measured and analysed. 3C. IHC staining and HE staining were performed on lymph node tissues in 3B with anti-Ovalbumin antibody. Ovalbumin positive cells were counted and analysed. Figure 3D. LN metastasis model was performed on C57BL/6J mice using LLC cells with knockdown of NADK. Popliteal lymph nodes were isolated at the end of the experiment, and LN volume was measured and analysed. 3E. Lymph node tissues in 3D were examined using IHC staining with anti-Ovalbumin antibody or H&E staining. Cells expressing Ovalbumin were counted and analysed. The scale bars were indicated. **, P<0.01.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Chicken

Applications

IHC-P, WB, ELISA, IP

applications

Immunogen

Native Full Length Protein corresponding to Chicken SERPINB14.

P01012

Reactivity data

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Product details

Product Specifications
Anti-Ovalbumin antibody (ab181688) is a rabbit polyclonal antibody and is validated for use in ELISA, IHC-P, IP, WB in chicken samples.
Anti-Ovalbumin antibody (ab181688) specifically detects Ovalbumin (UniProt ID: P01012; Molecular weight: 43kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Ovalbumin antibody (ab181688) has high sensitivity and specificity.

Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Non-inhibitory serpin. Storage protein of egg white.
See full target information SERPINB14

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 10:992-1005 PubMed40148566

2025

Immune targeting and host-protective effects of the latent stage of Toxoplasma gondii.

Applications

Unspecified application

Species

Unspecified reactive species

Julia N Eberhard,Lindsey A Shallberg,Aaron Winn,Sambamurthy Chandrasekaran,Christopher J Giuliano,Emily F Merritt,Elinor Willis,Christoph Konradt,David A Christian,Daniel L Aldridge,Molly E Bunkofske,Maxime Jacquet,Florence Dzierszinski,Eleni Katifori,Sebastian Lourido,Anita A Koshy,Christopher A Hunter

Cellular & molecular immunology 21:1251-1265 PubMed39164536

2024

Engineered mitochondria exert potent antitumor immunity as a cancer vaccine platform.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Luo,Fei Mo,Zhe Zhang,Weiqi Hong,Tianxia Lan,Yuan Cheng,Chunju Fang,Zhenfei Bi,Furong Qin,Jingyun Yang,Ziqi Zhang,Xue Li,Haiying Que,Jiayu Wang,Siyuan Chen,Yiming Wu,Li Yang,Jiong Li,Wei Wang,Chong Chen,Xiawei Wei

International journal of pharmaceutics 661:124404 PubMed38945464

2024

Boosting antigen-specific T cell activation with lipid-stabilized protein nanoaggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Schlich,Luciana D'Apice,Francesco Lai,Chiara Sinico,Donatella Valenti,Federico Catalano,Roberto Marotta,Paolo Decuzzi,Paola Italiani,Anna Maria Fadda

Proceedings of the National Academy of Sciences of the United States of America 121:e2322264121 PubMed38865265

2024

Transdermal microarrayed electroporation for enhanced cancer immunotherapy based on DNA vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Wang,Jin Qu,Chuxiao Xiong,Bing Chen,Kai Xie,Mingxue Wang,Zhen Liu,Zhao Yue,Zhenghua Liang,Feng Wang,Tianlong Zhang,Guangyu Zhu,Yi Becki Kuang,Peng Shi

Frontiers in immunology 14:1227633 PubMed37727784

2023

Targeted delivery of autoantigen to dendritic cells prevents development of spontaneous uveitis.

Applications

Unspecified application

Species

Unspecified reactive species

Izabela P Klaska,Tian Yu,Rosie Fordyce,Koju Kamoi,Richard J Cornall,Cristina Martin-Granados,Lucia Kuffova,John V Forrester

International journal of biological sciences 19:3184-3199 PubMed37416767

2023

Nicotinamide adenine dinucleotide kinase promotes lymph node metastasis of NSCLC via activating ID1 expression through BMP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zimei Zeng,Jie Gao,Tao Chen,Ziyu Zhang,Mengwei Li,Qi Fan,Guoqian Liu,Xuebing Li,Zhi Li,Chenxi Zhong,Feng Yao,Lunquan Sun,Yuezhen Deng,Min Li

Cancer cell 41:807-817.e6 PubMed37037617

2023

T cell immune deficiency rather than chromosome instability predisposes patients with short telomere syndromes to squamous cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen E Schratz,Diane A Flasch,Christine C Atik,Zoe L Cosner,Amanda L Blackford,Wentao Yang,Dustin L Gable,Paz J Vellanki,Zhimin Xiang,Valeriya Gaysinskaya,Robert H Vonderheide,Lisa M Rooper,Jinghui Zhang,Mary Armanios

Journal of Cancer 13:2126-2137 PubMed35517410

2022

BET inhibitor JQ1 enhances anti-tumor immunity and synergizes with PD-1 blockade in CRC.

Applications

Unspecified application

Species

Unspecified reactive species

Huijin Wang,Guangyao Liu,Xinghan Jin,Shenglei Song,Songyao Chen,Peiqing Zhou,Huan Li,Jianming Liang,Bo Li,Changhua Zhang,Yulong He

Scientific reports 12:5112 PubMed35332214

2022

Combining elemental and immunochemical analyses to characterize diagenetic alteration patterns in ancient skeletal remains.

Applications

Unspecified application

Species

Unspecified reactive species

L Gatti,Federico Lugli,Giorgia Sciutto,M Zangheri,S Prati,M Mirasoli,S Silvestrini,S Benazzi,T Tütken,K Douka,C Collina,F Boschin,M Romandini,P Iacumin,M Guardigli,A Roda,R Mazzeo

eLife 10: PubMed33843587

2021

Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon M Walsh,Ryan M Sheridan,Erin D Lucas,Thu A Doan,Brian C Ware,Johnathon Schafer,Rui Fu,Matthew A Burchill,Jay R Hesselberth,Beth Ann Jiron Tamburini
View all publications

Product promise

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