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Anti-Ovalbumin antibody [EPR27117-90] (ab306591) is a rabbit monoclonal antibody detecting Ovalbumin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Chicken.



- Biophysical QC for unrivalled batch-batch consistency

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Images

Western blot - Anti-Ovalbumin antibody [EPR27117-90] (AB306591), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ovalbumin antibody [EPR27117-90] (AB306591), expandable thumbnail
  • Immunocytochemistry - Anti-Ovalbumin antibody [EPR27117-90] (AB306591), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Chicken
Tested
Tested
Tested

Tested
Tested

Species
Chicken
Dilution info
1/1000
Notes

This antibody is only recommended in chicken. It may detect 70kDa and 40-50kDa nonspecific bands in cell lines of other species in highly sensitive testing system.

Tested
Tested

Species
Chicken
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Chicken
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Non-inhibitory serpin. Storage protein of egg white.

Alternative names

Recommended products

Anti-Ovalbumin antibody [EPR27117-90] (ab306591) is a rabbit monoclonal antibody detecting Ovalbumin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Chicken.



- Biophysical QC for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27117-90
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Ovalbumin antibody [EPR27117-90] (ab306591) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Chicken samples.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR27117-90] also available for your convenience:
ab306591, Carrier free - Anti-Ovalbumin antibody [EPR27117-90] - BSA and Azide free ab306592



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ovalbumin also known as egg albumin is a significant protein found in egg white representing about 54% of the protein content in this part of the egg. It has a molecular weight of approximately 45 kDa. This protein is synthesized in the oviduct of birds and deposited in the egg white where it fulfills several roles. Ovalbumin is a glycoprotein meaning it has carbohydrate groups attached to the polypeptide chain. Researchers commonly use it as a model antigen in immunology studies and as a standard in protein markers because of its known properties.

Biological function summary

Ovalbumin functions as a storage protein in eggs providing essential amino acids for the developing embryo. This protein also plays a role in binding metal ions and vitamins acting as a nutrient reservoir. It is not part of a complex existing as a single polypeptide chain. Ovalbumin contributes to the regulation and transport of metabolic compounds due to its ability to bind ions. These properties make it useful in various biological studies and industrial applications.

Pathways

Four significant pathways involve the role of ovalbumin in living organisms. These include the regulation of protein adsorption and the nutrient reservoir activity pathway. Ovalbumin's ability to serve as a nutrient source directly relates to these pathways ensuring proper nourishment during embryo development. It operates independently of other proteins in these pathways emphasizing its role in providing and regulating nutrients without the aid of additional complexes.

Associated diseases and disorders

Ovalbumin has connections to some allergic responses particularly in individuals with egg allergies. It acts as an allergen capable of provoking immune reactions. Ovalbumin lacks a direct connection to specific diseases beyond allergies. However understanding its interaction with immune system proteins like immunoglobulin E (IgE) is important as this is the primary mediator in allergic responses. This link makes ovalbumin important for research into allergy mechanisms and the development of hypoallergenic variants.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Ovalbumin antibody [EPR27117-90] (ab306591), expandable thumbnail

    Western blot - Anti-Ovalbumin antibody [EPR27117-90] (ab306591)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The band at approximately 80 kDa is expected to be Ovalbumin dimer (PMID: 30101285).
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-Ovalbumin antibody [EPR27117-90] (ab306591) at 1/1000 dilution

    Lane 1: 293T transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

    Lane 2: 293T transfected with a chicken Ovalbumin vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: Chicken Ovalbumin protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45 kDa

    Exposure time: 15s

  • Flow Cytometry (Intracellular) - Anti-Ovalbumin antibody [EPR27117-90] (ab306591), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ovalbumin antibody [EPR27117-90] (ab306591)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with a Ovalbumin expression vector containing a myc-his tag cells labelling Ovalbumin with ab306591 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry - Anti-Ovalbumin antibody [EPR27117-90] (ab306591), expandable thumbnail

    Immunocytochemistry - Anti-Ovalbumin antibody [EPR27117-90] (ab306591)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Ovalbumin with ab306591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with chicken Ovalbumin vector containing a myc tag. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red).
    The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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