Anti-Ovalbumin antibody [EPR27117-90]

• ICC

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Immunocytochemistry - Anti-Ovalbumin antibody [EPR27117-90] (AB306591)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Ovalbumin with ab306591 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with chicken Ovalbumin vector containing a myc tag. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Ovalbumin antibody [EPR27117-90] (AB306591)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with a Ovalbumin expression vector containing a myc-his tag cells labelling Ovalbumin with ab306591 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-Ovalbumin antibody [EPR27117-90] (AB306591)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The band at approximately 80 kDa is expected to be Ovalbumin dimer (PMID : 30101285). Exposure time : 15 seconds

All lanes:

Western blot - Anti-Ovalbumin antibody [EPR27117-90] (ab306591) at 1/1000 dilution

Lane 1:

293T transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T transfected with a chicken Ovalbumin vector containi a His-tag whole cell lysate at 20 µg

Lane 3:

Chicken Ovalbumin protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 15s