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AB250862

Anti-OVCA1 antibody [EPR15342] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal OVCA1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DPH2L, DPH2L1, OVCA1, DPH1, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1, Diphthamide biosynthesis protein 1, Diphtheria toxin resistance protein 1, Ovarian cancer-associated gene 1 protein, S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1

3 Images
Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • IP

Supplier Data

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/en-us/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>)

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/en-us/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>) at 1/10000 dilution

Lane 1:

SKBR3 cell lysate at 20 µg

Lane 2:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/en-us/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>) at 1/1000 dilution

Lane 1:

SK OV 3 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250862 is the carrier-free version of ab185960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OVCA1 also known as DPH1 or DPH2L1 is a protein encoded by a gene located in human chromosome 11p11.2. The protein weighs approximately 42 kDa. OVCA1 is involved in the early steps of diphthamide biosynthesis where it functions by interacting with its protein partners. It shows expression in various tissues with higher levels in the ovary and heart. OVCA1's role gets linked to cellular processes that impact cell growth and survival.
Biological function summary

This protein plays important roles in cellular proliferation and maintenance. OVCA1 is a part of the DPH1-DPH2 complex that facilitates the modification of eukaryotic elongation factor 2 (eEF2) through diphthamide synthesis. The process of 2-[3-carboxamido-3-(trimethylammonio)propyl]histidine diphthamide biosynthesis is essential for proper cellular protein translation. OVCA1 helps regulate the diphthamide biosynthesis pathway's first step thereby impacting the eEF2’s functionality which is important for protein synthesis and cell division.

Pathways

OVCA1's activity integrates into the cellular translation machinery where it influences the diphthamide biosynthesis pathway. This pathway is important for eEF2 modulation affecting translational control within cells. Another related pathway includes OVCA1's involvement in the cell cycle control mechanisms together with proteins like DPH2 and DPH3. By facilitating the modification of eEF2 OVCA1 effectively participates in the regulation of cellular growth impacting various growth regulatory pathways.

OVCA1 relates significantly to ovarian cancer and cardiomyopathy. Its dysfunction associates with the impaired regulation of cellular growth processes contributing to cancerous growths. OVCA1 interacts with p53 in ovarian cancer impacting tumor suppressor activities and cell cycle regulation. Additionally OVCA1 displays implications in cardiomyopathy potentially attributed to defective diphthamide biosynthesis and its downstream effects on cardiac tissue functionality. The OVCA1 and its network of associated proteins play an important part in fighting these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed : 30877278). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Acts as a tumor suppressor (PubMed : 10519411).
See full target information DPH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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