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AB185960

Anti-OVCA1 antibody [EPR15342] - C-terminal

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(3 Publications)

Rabbit Recombinant Monoclonal OVCA1 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

DPH2L, DPH2L1, OVCA1, DPH1, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1, Diphthamide biosynthesis protein 1, Diphtheria toxin resistance protein 1, Ovarian cancer-associated gene 1 protein, S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1

3 Images
Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)
  • IP

Supplier Data

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)

Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/10000 dilution

Lane 1:

SKBR3 cell lysate at 20 µg

Lane 2:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (AB185960)

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/1000 dilution

Lane 1:

SK OV 3 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

false

  • Carrier free

    Anti-OVCA1 antibody [EPR15342] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15342

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reagents, Controls & Accessories

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OVCA1 also known as DPH1 or DPH2L1 is a protein encoded by a gene located in human chromosome 11p11.2. The protein weighs approximately 42 kDa. OVCA1 is involved in the early steps of diphthamide biosynthesis where it functions by interacting with its protein partners. It shows expression in various tissues with higher levels in the ovary and heart. OVCA1's role gets linked to cellular processes that impact cell growth and survival.
Biological function summary

This protein plays important roles in cellular proliferation and maintenance. OVCA1 is a part of the DPH1-DPH2 complex that facilitates the modification of eukaryotic elongation factor 2 (eEF2) through diphthamide synthesis. The process of 2-[3-carboxamido-3-(trimethylammonio)propyl]histidine diphthamide biosynthesis is essential for proper cellular protein translation. OVCA1 helps regulate the diphthamide biosynthesis pathway's first step thereby impacting the eEF2’s functionality which is important for protein synthesis and cell division.

Pathways

OVCA1's activity integrates into the cellular translation machinery where it influences the diphthamide biosynthesis pathway. This pathway is important for eEF2 modulation affecting translational control within cells. Another related pathway includes OVCA1's involvement in the cell cycle control mechanisms together with proteins like DPH2 and DPH3. By facilitating the modification of eEF2 OVCA1 effectively participates in the regulation of cellular growth impacting various growth regulatory pathways.

OVCA1 relates significantly to ovarian cancer and cardiomyopathy. Its dysfunction associates with the impaired regulation of cellular growth processes contributing to cancerous growths. OVCA1 interacts with p53 in ovarian cancer impacting tumor suppressor activities and cell cycle regulation. Additionally OVCA1 displays implications in cardiomyopathy potentially attributed to defective diphthamide biosynthesis and its downstream effects on cardiac tissue functionality. The OVCA1 and its network of associated proteins play an important part in fighting these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed : 30877278). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Acts as a tumor suppressor (PubMed : 10519411).
See full target information DPH1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3301 PubMed38671004

2024

Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Shi,Daochao Huang,Cui Song,Ruixue Cao,Zhao Wang,Dan Wang,Li Zhao,Xiaolu Xu,Congyu Lu,Feng Xiong,Haowen Zhao,Shuxiang Li,Quansheng Zhou,Shuyue Luo,Dongjie Hu,Yun Zhang,Cui Wang,Yiping Shen,Weiting Su,Yili Wu,Karl Schmitz,Shuo Wei,Weihong Song

The Journal of clinical investigation 129:5005-5019 PubMed31437130

2019

DNA methyltransferase inhibition overcomes diphthamide pathway deficiencies underlying CD123-targeted treatment resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiro Togami,Timothy Pastika,Jason Stephansky,Mahmoud Ghandi,Amanda L Christie,Kristen L Jones,Carl A Johnson,Ross W Lindsay,Christopher L Brooks,Anthony Letai,Jeffrey W Craig,Olga Pozdnyakova,David M Weinstock,Joan Montero,Jon C Aster,Cory M Johannessen,Andrew A Lane

Cellular physiology and biochemistry : internation 44:505-514 PubMed29145210

2017

Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Minghui Liu,Kai Yin,Xu Guo,Huijin Feng,Min Yuan,Yanqing Liu,Jianguo Zhang,Baoliang Guo,Chen Wang,Guangxin Zhou,Zhen Zhou,Chen-Yu Zhang,Xi Chen
View all publications
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Product promise

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