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Rabbit Recombinant Monoclonal OX40L/TNFSF4 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (AB263910), expandable thumbnail
  • Flow Cytometry - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (AB263910), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (AB263910), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Alternative names

CD252, TXGP1, TNFSF4, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, TAX transcriptionally-activated glycoprotein 1, OX40L

Recommended products

Rabbit Recombinant Monoclonal OX40L/TNFSF4 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23155-317
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.

Biological function summary

OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.

Pathways

OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.

Associated diseases and disorders

OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910), expandable thumbnail

    Immunoprecipitation - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)

    OX40L/TNFSF4 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg with ab263910 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263910 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg

    Lane 2: ab263910 IP in HUVEC whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab263910 in HUVEC whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 20 seconds.

    This antibody failed in WB test due to weak signals. But it can detect clear band after IP enrichment.

    All lanes: Immunoprecipitation - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)

    Predicted band size: 21 kDa

  • Flow Cytometry - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910), expandable thumbnail

    Flow Cytometry - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)

    Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling CD252/OX40L with ab263910 at 0.002 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263910).

  • Immunocytochemistry/ Immunofluorescence - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling CD252/OX40L with ab263910 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and �staining in HUVEC cell line Negative control: HeLa (PMID: 1996093) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263910).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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