Rabbit Recombinant Monoclonal OX40L/TNFSF4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (AB275858), expandable thumbnail](https://content.abcam.com/products/images/ox40l-tnfsf4-antibody-epr23155-317-bsa-and-azide-free-capture-ab275858--sandwich-elisa-img425726.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (AB275858)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Alternative names
TXGP1, TNFSF4, TXGP1, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, TAX transcriptionally-activated glycoprotein 1, OX40L
Recommended products
Rabbit Recombinant Monoclonal OX40L/TNFSF4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23155-317
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
ab275858 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TNFSF4. The recommended pair for sandwich ELISA is:
Capture: ab275858, Human TNFSF4 Capture Antibody (unconjugated)
Detector: Anti-OX40L/TNFSF4 antibody [EPR23155-12] - BSA and Azide free (Detector) ab275856, Human TNFSF4 Detector Antibody (unconjugated)
The reference range value is 58.59-7500 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.
- Biological function summary
OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.
- Pathways
OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.
- Associated diseases and disorders
OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (ab275858), expandable thumbnail](https://content.abcam.com/products/images/ox40l-tnfsf4-antibody-epr23155-317-bsa-and-azide-free-capture-ab275858--sandwich-elisa-img425726.jpg "Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (ab275858)")
Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (ab275858)
Sandwich ELISA of Human TNFSF4 Antibody Pair - BSA and Azide free ab253549 with the capture antibody ab275858 dilution at 2 µg/mL and detector antibody Anti-OX40L/TNFSF4 antibody [EPR23155-12] - BSA and Azide free (Detector) ab275856 dilution at 0.5 µg/mL.
Downloads
Product protocols
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