AB105320

Anti-OXCT1/SCOT antibody

Name: Anti-OXCT1/SCOT antibody (ab105320) | Abcam
Brand: Abcam Limited
SKU: AB105320
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal OXCT1/SCOT antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OXCT1 aa 150-450.

View Alternative Names

OXCT, SCOT, OXCT1, 3-oxoacid CoA-transferase 1, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, Succinyl-CoA:3-oxoacid CoA transferase, SCOT-s

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (AB105320)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (AB105320)

ab105320 at 1/100 dilution staining OXCT1/SCOT in Human fetal kidney tissue

Unknown

Western blot - Anti-OXCT1/SCOT antibody (AB105320)

All lanes:

Western blot - Anti-OXCT1/SCOT antibody (ab105320) at 1/500 dilution

Lane 1:

Human fetal heart lysate

Lane 2:

Human fetal brain lysate

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human OXCT1 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P55809

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OXCT1 also known as SCOT (succinyl-CoA:3-ketoacid CoA transferase) is an enzyme that transfers CoA groups from succinyl-CoA to acetoacetate facilitating ketone body metabolism. This enzyme has a molecular mass of approximately 56 kDa. It is expressed widely in tissues with high metabolic activity including heart kidney and skeletal muscle where energy demands are significant. There OXCT1 aids in the efficient utilization of energy resources during processes like fasting and high-intensity exercise.

Biological function summary

OXCT1 functions as an important enzyme in ketolysis which is the breakdown of ketone bodies for energy production. It does not operate within a large complex but interacts closely with other enzymes involved in similar metabolic processes. OXCT1 ensures that tissues efficiently convert ketone bodies like acetoacetate and beta-hydroxybutyrate into acetyl-CoA which then feeds into the citric acid cycle to fulfill energy requirements especially when carbohydrates are sparse.

Pathways

OXCT1 is an important component of the ketone body metabolism pathway linking it to energy homeostasis and ketogenesis. It directly interacts with enzymes like acetoacetyl-CoA thiolase and beta-hydroxybutyrate dehydrogenase. Through these associations it integrates into larger metabolic pathways such as the citric acid cycle which is essential for energy production under conditions when lipids become a primary source for energy generation.

OXCT1 is implicated in conditions like ketosis-prone type 2 diabetes and mitochondrial diseases. Alterations in the enzyme's function can disrupt energy homeostasis leading to an excess of ketone bodies in the blood known as ketoacidosis particularly in diabetic patients. Additionally mutations or deficiencies in OXCT1 can be associated with disorders of mitochondrial energy metabolism with potential interactions involving proteins like pyruvate dehydrogenase and other components of the respiratory chain contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (PubMed : 10964512). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).

See full target information OXCT1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe

Journal of the American Heart Association 10:e020729 PubMed34583524

2021

Ketone Ester D-β-Hydroxybutyrate-(R)-1,3 Butanediol Prevents Decline in Cardiac Function in Type 2 Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Phung N Thai,Charles V Miller,M Todd King,Saul Schaefer,Richard L Veech,Nipavan Chiamvimonvat,Donald M Bers,Elena N Dedkova

Bioengineered 12:6559-6571 PubMed34559577

2021

Orthodenticle homeobox OTX1 is a potential prognostic biomarker for bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Jiang,Zhongqiang Zuo,Jie Lin,Chuanfeng Yang

Journal of neuroscience methods 334:108591 PubMed31926203

2020

Multiplex LA-ICP-MS bio-imaging of brain tissue of a parkinsonian mouse model stained with metal-coded affinity-tagged antibodies and coated with indium-spiked commercial inks as internal standards.

Applications

Unspecified application

Species

Unspecified reactive species

Boris Neumann,Simone Hösl,Karima Schwab,Franz Theuring,Norbert Jakubowski

PloS one 14:e0210454 PubMed31034522

2019

miRNAs, target genes expression and morphological analysis on the heart in gestational protein-restricted offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Heloisa Balan Assalin,José Antonio Rocha Gontijo,Patrícia Aline Boer

Stem cell reviews and reports 15:427-438 PubMed30338499

2018

Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Aghajani Nargesi,Xiang-Yang Zhu,LaTonya J Hickson,Sabena M Conley,Andre J van Wijnen,Lilach O Lerman,Alfonso Eirin

American journal of physiology. Endocrinology and 312:E437-E446 PubMed28223292

2017

Muscle PGC-1α is required for long-term systemic and local adaptations to a ketogenic diet in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Svenia Schnyder,Kristoffer Svensson,Bettina Cardel,Christoph Handschin

FASEB journal : official publication of the Federa 30:1976-86 PubMed26849960

2016

Skeletal muscle PGC-1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristoffer Svensson,Verena Albert,Bettina Cardel,Silvia Salatino,Christoph Handschin

American journal of physiology. Endocrinology and 304:E826-35 PubMed23423173

2013

Metallothionein prevents diabetes-induced cardiac pathological changes, likely via the inhibition of succinyl-CoA:3-ketoacid coenzyme A transferase-1 nitration at Trp(374).

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Cong,Weide Ma,Ting Zhao,Zhongxin Zhu,Yuehui Wang,Yi Tan,Xiaokun Li,Litai Jin,Lu Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-OXCT1/SCOT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (AB105320)

Western blot - Anti-OXCT1/SCOT antibody (AB105320)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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