Rabbit Polyclonal OXCT1/SCOT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OXCT1 aa 50-250.
Images
Publications
Filter by
- Frontiers in endocrinology 13:9608352022Hyperinsulinemia impairs the metabolic switch to ketone body utilization in proximal renal tubular epithelial cells under energy crisis the inhibition of the SIRT3/SMCT1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinlan Xie,Feifei Zhong,Zhenhong Guo,Xinran Li,Jingyu Wang,Zhongai Gao,Baocheng Chang,Juhong YangPubMed 36237185
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OXCT1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P55809
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Pig | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (PubMed:10964512). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).
Alternative names
OXCT, SCOT, OXCT1, 3-oxoacid CoA-transferase 1, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, Succinyl-CoA:3-oxoacid CoA transferase, SCOT-s
Recommended products
Rabbit Polyclonal OXCT1/SCOT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OXCT1 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OXCT1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P55809
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OXCT1 also known as SCOT (succinyl-CoA:3-ketoacid CoA transferase) is an enzyme that transfers CoA groups from succinyl-CoA to acetoacetate facilitating ketone body metabolism. This enzyme has a molecular mass of approximately 56 kDa. It is expressed widely in tissues with high metabolic activity including heart kidney and skeletal muscle where energy demands are significant. There OXCT1 aids in the efficient utilization of energy resources during processes like fasting and high-intensity exercise.
- Biological function summary
OXCT1 functions as an important enzyme in ketolysis which is the breakdown of ketone bodies for energy production. It does not operate within a large complex but interacts closely with other enzymes involved in similar metabolic processes. OXCT1 ensures that tissues efficiently convert ketone bodies like acetoacetate and beta-hydroxybutyrate into acetyl-CoA which then feeds into the citric acid cycle to fulfill energy requirements especially when carbohydrates are sparse.
- Pathways
OXCT1 is an important component of the ketone body metabolism pathway linking it to energy homeostasis and ketogenesis. It directly interacts with enzymes like acetoacetyl-CoA thiolase and beta-hydroxybutyrate dehydrogenase. Through these associations it integrates into larger metabolic pathways such as the citric acid cycle which is essential for energy production under conditions when lipids become a primary source for energy generation.
- Associated diseases and disorders
OXCT1 is implicated in conditions like ketosis-prone type 2 diabetes and mitochondrial diseases. Alterations in the enzyme's function can disrupt energy homeostasis leading to an excess of ketone bodies in the blood known as ketoacidosis particularly in diabetic patients. Additionally mutations or deficiencies in OXCT1 can be associated with disorders of mitochondrial energy metabolism with potential interactions involving proteins like pyruvate dehydrogenase and other components of the respiratory chain contributing to disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-OXCT1/SCOT antibody (ab224250)
Loading control: Anti-COX4I1.
Lane 1: ab224250 at 0.4 µg/mL
Lane 2: Western blot - Anti-OXCT1/SCOT antibody (ab224250) at 0.4 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2: A549 (Human lung carcinoma cell line) lysate
Predicted band size: 56 kDa
Western blot - Anti-OXCT1/SCOT antibody (ab224250)
All lanes: Western blot - Anti-OXCT1/SCOT antibody (ab224250) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2: NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 56 kDa, 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (ab224250)
Paraffin-embedded human testis stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (ab224250)
Paraffin-embedded human heart stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cardiomyocytes.
Western blot - Anti-OXCT1/SCOT antibody (ab224250)
False colour image of Western blot: Anti-OXCT1/SCOT antibody staining at 0.04 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab224250 was shown to bind specifically to OXCT1/SCOT. A band was observed at 56 kDa in wild-type HeLa cell lysates with no signal observed at this size in OXCT1 knockout cell line Human OXCT1 (SCOT) knockout HeLa cell line ab265358 (knockout cell lysate Human OXCT1 (SCOT) knockout HeLa cell lysate ab258557). To generate this image, wild-type and OXCT1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-OXCT1/SCOT antibody (Anti-OXCT1/SCOT antibody ab241125) at 1/10000 dilution
Lanes 1 - 4: Western blot - Anti-OXCT1/SCOT antibody (Anti-OXCT1/SCOT antibody ab241221) at 1/10000 dilution
Lanes 1 - 4: Western blot - Anti-OXCT1/SCOT antibody (ab224250) at 0.04 µg/mL
Lane 1: Wild-type HeLa cell lysate at 18 µg
Lane 2: OXCT1 knockout HeLa cell lysate at 18 µg
Lane 2: Western blot - Human OXCT1 (SCOT) knockout HeLa cell line (Human OXCT1 (SCOT) knockout HeLa cell line ab265358)
Lane 3: Jurkat cell lysate at 18 µg
Lane 4: A549 cell lysate at 18 µg
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (ab224250)
Paraffin-embedded human liver stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXCT1/SCOT antibody (ab224250)
Paraffin-embedded human kidney stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in tubules.
Immunocytochemistry/ Immunofluorescence - Anti-OXCT1/SCOT antibody (ab224250)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for OXCT1/SCOT (green) using ab224250 (4 μg/ml) in ICC/IF.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com