AB229111

Anti-OXSM antibody

Name: Anti-OXSM antibody (ab229111) | Abcam
Brand: Abcam Limited
SKU: AB229111

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal OXSM antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OXSM.

View Alternative Names

Beta-ketoacyl-ACP synthase, OXSM

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSM antibody (AB229111)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSM antibody (AB229111)

Paraffin-embedded human colon carcinoma tissue stained for OXSM using ab229111 at 1/250 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSM antibody (AB229111)

Paraffin-embedded human mouse muscle tissue stained for OXSM using ab229111 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-OXSM antibody (AB229111)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-OXSM antibody (ab229111) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 28 kDa,48 kDa,57 kDa

Observed band size: 42 kDa,57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human OXSM. The exact immunogen used to generate this antibody is proprietary information.

Q9NWU1

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OXSM also known as 3-oxoacyl-[acyl-carrier-protein] synthase mitochondrial is an important enzyme in the mitochondrial fatty acid synthesis pathway. It participates in the elongation of fatty acids by catalyzing a ketoacyl synthesis reaction. This protein has a molecular mass of approximately 50 kDa. OXSM is mainly expressed in tissues with high metabolic activity such as liver and muscle tissues where it supports the dynamic balance of fatty acid synthesis.

Biological function summary

OXSM plays a part in mitochondrial lipid homeostasis by facilitating the synthesis of longer chain fatty acids. It operates as part of a multiprotein complex that ensures proper substrate channeling and processing. This function is critical for the maintenance and functioning of cellular membranes providing the necessary lipid components. Additionally OXSM contributes to energy production by generating fatty acids that can be converted into acetyl-CoA units within the mitochondria.

Pathways

Several metabolic pathways involve OXSM including the mitochondrial fatty acid synthesis and the broader lipid metabolism pathway. In the mitochondrial fatty acid synthesis pathway OXSM interacts with proteins like Acyl-CoA synthetase family member 1 (ACSF1) to facilitate the progression of fatty acid synthesis. These pathways collectively contribute to energy homeostasis and cellular membrane composition reflecting the interconnectivity between various metabolic functions.

Researchers associate OXSM with rare mitochondrial disorders linked to lipid metabolism. For instance mutations or dysfunctions in OXSM can result in compromised mitochondrial function potentially contributing to neuromuscular disorders. It also connects to carnitine palmitoyltransferase 1A (CPT1A) another key enzyme in fatty acid metabolism which highlights the interconnected nature of mitochondrial metabolic disorders. Understanding OXSM's role in these conditions can lead to better insights for therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.

See full target information OXSM

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC oral health 22:126 PubMed35428233

2022

Identification and validation of transcription factor-driven enhancers of genes related to lipid metabolism in metastatic oral squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Liru Zhang,Shuangling Zhao,Yuanhang Liu,Feifei Lv,Xixue Geng

FEBS letters 595:2271-2289 PubMed34328660

2021

Genome-wide analysis of the mouse LIM gene family reveals its roles in regulating pathological cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Wang,Jieqiong Zhao,Mingming Zhang,Jingxiao Yang,Guangwei Zeng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OXSM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSM antibody (AB229111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSM antibody (AB229111)

Western blot - Anti-OXSM antibody (AB229111)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise