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AB224248

Anti-OXSR1 antibody

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(2 Publications)

Rabbit Polyclonal OXSR1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OXSR1 aa 300-500.

View Alternative Names

KIAA1101, OSR1, OXSR1, Serine/threonine-protein kinase OSR1, Oxidative stress-responsive 1 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 antibody (AB224248)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 antibody (AB224248)

Paraffin embedded human esophagus tissue stained for OXSR1 with ab224248 (1/50 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-OXSR1 antibody (AB224248)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OXSR1 antibody (AB224248)

PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for OXSR1 (green) using ab224248 (4 μg/ml) in ICC/IF.

Western blot - Anti-OXSR1 antibody (AB224248)
  • WB

Supplier Data

Western blot - Anti-OXSR1 antibody (AB224248)

All lanes:

Western blot - Anti-OXSR1 antibody (ab224248) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human OXSR1 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

O95747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OXSR1 also known as oxidative stress response 1 or OSR1 is a serine/threonine kinase with a mass of approximately 58 kDa. It plays a mechanical role in regulating cellular responses to environmental stress especially oxidative stress. OXSR1 is expressed in several tissues including the heart kidney and brain indicating its importance across different physiological contexts. The protein functions by activating downstream substrates involved in cellular protection and homeostasis.
Biological function summary

Oxidative stress response kinase 1 participates in cellular defense mechanisms against oxidative damage. OXSR1 does not form part of a larger complex but has unique roles in signaling pathways helping to defend cells from oxidative stress. This protein kinase modulates ion channels and transporters participating in processes that stabilize the cellular environment and maintain ionic balance important for cell survival under stress conditions.

Pathways

OXSR1 plays an important role in the oxidative stress response and the MAPK signaling pathway. The protein interacts with and is regulated by STE20 family kinases including SPAK which are partners in regulating cellular stress responses. These pathways involve phosphorylation events leading to changes in cellular activity therefore supporting adaption to stressful conditions. OXSR1 acts as a mediator in connecting upstream signals to downstream cellular effects.

Oxidative stress response kinase 1 is associated with hypertension and neurodegenerative diseases. Variations or dysfunction in OXSR1 can disrupt ion homeostasis and cellular stress response contributing to the pathogenesis of these conditions. The kinase's interaction with SPAK is significant in these disorders where altered activity influences blood pressure regulation and neuronal survival. Studies continue investigating its potential role in therapeutic interventions for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed : 16669787, PubMed : 18270262, PubMed : 21321328, PubMed : 34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed : 16669787, PubMed : 17721439, PubMed : 21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4) : following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 17721439). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed : 16669787, PubMed : 21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed : 16669787, PubMed : 19665974, PubMed : 21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed : 18270262). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (PubMed : 23386621, PubMed : 25362046). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1 : recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (PubMed : 29581290). Phosphorylates RELL1, RELL2 and RELT (PubMed : 16389068, PubMed : 28688764). Phosphorylates PAK1 (PubMed : 14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed : 22052202).
See full target information OXSR1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6035 PubMed34654800

2021

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Stangherlin,Joseph L Watson,David C S Wong,Silvia Barbiero,Aiwei Zeng,Estere Seinkmane,Sew Peak Chew,Andrew D Beale,Edward A Hayter,Alina Guna,Alison J Inglis,Marrit Putker,Eline Bartolami,Stefan Matile,Nicolas Lequeux,Thomas Pons,Jason Day,Gerben van Ooijen,Rebecca M Voorhees,David A Bechtold,Emmanuel Derivery,Rachel S Edgar,Peter Newham,John S O'Neill

Journal of Cancer 12:6563-6575 PubMed34659547

2021

Quantitative proteomic analysis reveals sophisticated metabolic alteration and identifies FMNL1 as a prognostic marker in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Ma,Zirui Wang,Junyao Liu,Shengjun Fu,Lili Zhang,Duo Zheng,Panfeng Shang,Zhongjin Yue
View all publications
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Product promise

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