AB138655

Anti-OXSR1 (phospho T185) antibody

Name: Anti-OXSR1 (phospho T185) antibody (ab138655) | Abcam
Brand: Abcam Limited
SKU: AB138655

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(2 Publications)

Rabbit Polyclonal OXSR1 phospho T185 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA1101, OSR1, OXSR1, Serine/threonine-protein kinase OSR1, Oxidative stress-responsive 1 protein

1 Images

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Western blot - Anti-OXSR1 (phospho T185) antibody (AB138655)

All lanes:

Western blot - Anti-OXSR1 (phospho T185) antibody (ab138655) at 1/500 dilution

All lanes:

HepG2 cell extract (treated with serum 20% for 15 minutes) at 30 µg

Predicted band size: 58 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138665 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OXSR1 also known as oxidative stress response 1 or OSR1 is a serine/threonine kinase with a mass of approximately 58 kDa. It plays a mechanical role in regulating cellular responses to environmental stress especially oxidative stress. OXSR1 is expressed in several tissues including the heart kidney and brain indicating its importance across different physiological contexts. The protein functions by activating downstream substrates involved in cellular protection and homeostasis.

Biological function summary

Oxidative stress response kinase 1 participates in cellular defense mechanisms against oxidative damage. OXSR1 does not form part of a larger complex but has unique roles in signaling pathways helping to defend cells from oxidative stress. This protein kinase modulates ion channels and transporters participating in processes that stabilize the cellular environment and maintain ionic balance important for cell survival under stress conditions.

Pathways

OXSR1 plays an important role in the oxidative stress response and the MAPK signaling pathway. The protein interacts with and is regulated by STE20 family kinases including SPAK which are partners in regulating cellular stress responses. These pathways involve phosphorylation events leading to changes in cellular activity therefore supporting adaption to stressful conditions. OXSR1 acts as a mediator in connecting upstream signals to downstream cellular effects.

Oxidative stress response kinase 1 is associated with hypertension and neurodegenerative diseases. Variations or dysfunction in OXSR1 can disrupt ion homeostasis and cellular stress response contributing to the pathogenesis of these conditions. The kinase's interaction with SPAK is significant in these disorders where altered activity influences blood pressure regulation and neuronal survival. Studies continue investigating its potential role in therapeutic interventions for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed : 16669787, PubMed : 18270262, PubMed : 21321328, PubMed : 34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed : 16669787, PubMed : 17721439, PubMed : 21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4) : following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 17721439). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed : 16669787, PubMed : 21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed : 16669787, PubMed : 19665974, PubMed : 21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed : 18270262). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (PubMed : 23386621, PubMed : 25362046). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1 : recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (PubMed : 29581290). Phosphorylates RELL1, RELL2 and RELT (PubMed : 16389068, PubMed : 28688764). Phosphorylates PAK1 (PubMed : 14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed : 22052202).

See full target information OXSR1 phospho T185

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:506 PubMed40319271

2025

The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhao,Xin Chen,Yanan Zhang,Yixuan Cen,Tingjia Zhu,Lingfang Wang,Lili Xia,Yang Li,Xiaodong Cheng,Xing Xie,Weiguo Lu,Junfen Xu

Nature communications 12:6035 PubMed34654800

2021

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Stangherlin,Joseph L Watson,David C S Wong,Silvia Barbiero,Aiwei Zeng,Estere Seinkmane,Sew Peak Chew,Andrew D Beale,Edward A Hayter,Alina Guna,Alison J Inglis,Marrit Putker,Eline Bartolami,Stefan Matile,Nicolas Lequeux,Thomas Pons,Jason Day,Gerben van Ooijen,Rebecca M Voorhees,David A Bechtold,Emmanuel Derivery,Rachel S Edgar,Peter Newham,John S O'Neill

View all publications

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Product promise

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Anti-OXSR1 (phospho T185) antibody

Western blot - Anti-OXSR1 (phospho T185) antibody (AB138655)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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