AB192803

Anti-OXSR1 (phospho T185) antibody

Name: Anti-OXSR1 (phospho T185) antibody (ab192803) | Abcam
Brand: Abcam Limited
SKU: AB192803
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal OXSR1 phospho T185 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OXSR1 pT185 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA1101, OSR1, OXSR1, Serine/threonine-protein kinase OSR1, Oxidative stress-responsive 1 protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 (phospho T185) antibody (AB192803)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 (phospho T185) antibody (AB192803)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling OXSR1 (phospho T185) with ab192803 at 1/50 (left) or ab192803 preincubated with blocking peptide (right).

Supplier Data

Western blot - Anti-OXSR1 (phospho T185) antibody (AB192803)

All lanes:

Western blot - Anti-OXSR1 (phospho T185) antibody (ab192803) at 1/500 dilution

Lane 1:

HepG2 cell extract

Lane 2:

HepG2 cell extract with antigen-specific peptide

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human OXSR1 pT185 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O95747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192803 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OXSR1 also known as oxidative stress response 1 or OSR1 is a serine/threonine kinase with a mass of approximately 58 kDa. It plays a mechanical role in regulating cellular responses to environmental stress especially oxidative stress. OXSR1 is expressed in several tissues including the heart kidney and brain indicating its importance across different physiological contexts. The protein functions by activating downstream substrates involved in cellular protection and homeostasis.

Biological function summary

Oxidative stress response kinase 1 participates in cellular defense mechanisms against oxidative damage. OXSR1 does not form part of a larger complex but has unique roles in signaling pathways helping to defend cells from oxidative stress. This protein kinase modulates ion channels and transporters participating in processes that stabilize the cellular environment and maintain ionic balance important for cell survival under stress conditions.

Pathways

OXSR1 plays an important role in the oxidative stress response and the MAPK signaling pathway. The protein interacts with and is regulated by STE20 family kinases including SPAK which are partners in regulating cellular stress responses. These pathways involve phosphorylation events leading to changes in cellular activity therefore supporting adaption to stressful conditions. OXSR1 acts as a mediator in connecting upstream signals to downstream cellular effects.

Oxidative stress response kinase 1 is associated with hypertension and neurodegenerative diseases. Variations or dysfunction in OXSR1 can disrupt ion homeostasis and cellular stress response contributing to the pathogenesis of these conditions. The kinase's interaction with SPAK is significant in these disorders where altered activity influences blood pressure regulation and neuronal survival. Studies continue investigating its potential role in therapeutic interventions for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed : 16669787, PubMed : 18270262, PubMed : 21321328, PubMed : 34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed : 16669787, PubMed : 17721439, PubMed : 21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4) : following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 17721439). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed : 16669787, PubMed : 21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed : 16669787, PubMed : 19665974, PubMed : 21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed : 18270262). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (PubMed : 23386621, PubMed : 25362046). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1 : recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (PubMed : 29581290). Phosphorylates RELL1, RELL2 and RELT (PubMed : 16389068, PubMed : 28688764). Phosphorylates PAK1 (PubMed : 14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed : 22052202).

See full target information OXSR1 pT185

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 20:7 PubMed34983556

2022

Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.

Applications

Unspecified application

Species

Unspecified reactive species

Pegah Zanjanchi,S Mohsen Asghari,Hassan Mohabatkar,Mostafa Shourian,Mehdi Shafiee Ardestani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-OXSR1 (phospho T185) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 (phospho T185) antibody (AB192803)

Western blot - Anti-OXSR1 (phospho T185) antibody (AB192803)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise