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AB212193

Anti-Oxytocin-neurophysin 1 antibody [EPR20973]

4

(1 Review)

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(17 Publications)

Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) is a rabbit monoclonal antibody detecting Oxytocin-neurophysin 1 in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

OT, OXT, Oxytocin-neurophysin 1, OT-NPI

5 Images
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID : 24464043). The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID : 24464043). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID : 24464043). The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID : 24464043). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)
  • WB

Supplier Data

Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)

Exposure times : Lane 1 : 3 seconds; Lane 2 : 1 second.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) at 1/1000 dilution

Lane 1:

Mouse pituitary tissue lysate at 10 µg

Lane 2:

Rat pituitary tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 12 kDa

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  • Carrier free

    Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20973

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific expression in pituitary tissue in WB and posterior lobe in IHC.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

What is the molecular weight of Oxytocin-neurophysin 1?
Anti-Oxytocin-neurophysin 1 [EPR20973] (ab212193) specifically detects a band for Oxytocin-neurophysin 1 (UniProt: P01178) at a molecular weight of 12kDa.

Trusted by the scientific community
Anti-Oxytocin-neurophysin 1 [EPR20973] (ab212193) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20973] also available for your convenience: ab212193, Carrier free - ab226490

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxytocin-neurophysin 1 also known as prepro-oxytocin-neurophysin I is a precursor protein with a molecular weight of approximately 20 kDa. This protein is expressed primarily in the hypothalamus and is involved in the synthesis and storage of the hormone oxytocin a peptide essential for various physiological processes. During synthesis the protein undergoes cleavage to separate oxytocin and neurophysin 1 a carrier protein that aids in the transport and stabilization of oxytocin in neurosecretory granules.
Biological function summary

Oxytocin-neurophysin 1 plays an important role in the regulation of social behaviors reproductive functions and fluid homeostasis. This precursor is not part of a larger protein complex but neurophysin 1 serves as an essential carrier that binds and transports oxytocin to its target sites in the body. Oxytocin itself functions as a neuromodulator and hormone exerting effects on the uterine muscles and mammary glands promoting childbirth and lactation.

Pathways

The biosynthesis and release of oxytocin-neurophysin 1 are closely associated with the hypothalamic neurosecretory pathways. These pathways involve the neurohypophysis where oxytocin is released into the bloodstream. Additionally oxytocin interacts with the G-protein-coupled receptor OXTR to elicit its physiological effects. V1a and V2 receptors alongside oxytocin play roles in various signaling pathways that affect metabolic and cardiovascular functions.

Dysregulation of oxytocin-neurophysin 1 has been linked to the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and autism spectrum disorders. In these conditions altered levels of oxytocin affect fluid balance and social behavior. Additionally changes in oxytocin levels often in tandem with altered vasopressin regulation associate with the development and exacerbation of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurophysin 1 specifically binds oxytocin.. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PubMed : 18174156).
See full target information OXT

Additional targets

Oxytocin-neurophysin 1
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 26:179 PubMed40770605

2025

Compromised PGF2α signaling in the paraventricular hypothalamic nucleus contributes to the central sensitization of the nitroglycerin-induced chronic migraine in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xianrong Hu,Lunquan Wu,Li Wang,Congxue Peng,Yunyun Tian,Qian Zhu,Hongwei Zhu,Liang Nie,Li Yin,Yuehui Zhang,Jiang Bian

CNS neuroscience & therapeutics 31:e70465 PubMed40468614

2025

The Oxytocin Neurons in the Paraventricular Nucleus Are Essential for Chronic Sleep Deprivation-Mediated Anxiety-Related Behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Wang,Yifei Zhang,Yanchao Liu,Zhendong Xu,Jiyan Zhang,Like Wang,Yufeng Cang,Junbin Xin,Fuyu Han,Zhouhua Li,Chuanwei Hu,Xiangjie Kong,Yuchen Deng,Li Zhang,Hairong Wang,Haibo Xu,Ming Chen,Linlin Bi

Actas espanolas de psiquiatria 53:504-515 PubMed40356008

2025

Activation of the Oxytocin System in the Hypothalamic Paraventricular Nucleus Improves Stress-Induced Postpartum Depression-Like Behavior in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Dang,Huihui Kuai,Siqi Zhou,Shanshan Guo,Jingyi Sheng,Zhiping Wang

Neuroendocrinology 115:564-575 PubMed40222363

2025

Acute Optogenetic Activation of the Subfornical Organ Produces Sympathetically Mediated Increases in Blood Pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Monique L Van Acquoy,Teodora Nedelkoska,Simon McMullan,Peter G R Burke,Cara M Hildreth

Neuroendocrinology 114:1090-1111 PubMed39348823

2024

Intranasal Application of Diluted Saline Alleviates Ischemic Brain Injury in Association with Suppression of Vasopressin Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Hou,Guichuan Chen,Di Li,Xiaoran Wang,Xiaoyu Liu,Dan Cui,Yunhao Jiang,Yang Liu,Ping Wang,Yu-Feng Wang,Dexin Meng,Shuwei Jia

Translational psychiatry 14:208 PubMed38796566

2024

Oxytocin attenuates hypothalamic injury-induced cognitive dysfunction by inhibiting hippocampal ERK signaling and Aβ deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Guangsen Wu,Yichao Ou,Zhanpeng Feng,Zhiwei Xiong,Kai Li,Mengjie Che,Songtao Qi,Mingfeng Zhou

Journal of virology 97:e0100623 PubMed37732785

2023

Zika virus infection leads to hormone deficiencies of the hypothalamic-pituitary-gonadal axis and diminished fertility in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Bo Liu,Wei Yang,Jia-Tong Chang,Dong-Ying Fan,Yan-Hua Wu,Pei-Gang Wang,Jing An

CNS neuroscience & therapeutics 29:3493-3506 PubMed37248645

2023

Paraventricular nucleus-central amygdala oxytocinergic projection modulates pain-related anxiety-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Li,Wei-Jia Du,Rui Liu,Gui-Ying Zan,Bing-Lu Ye,Qian Li,Zhi-Hao Sheng,Ya-Wei Yuan,Yu-Jie Song,Jing-Gen Liu,Zhi-Qiang Liu

Molecular psychiatry 27:4948-4958 PubMed36138127

2022

Leptin enhances social motivation and reverses chronic unpredictable stress-induced social anhedonia during adolescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Lei,Dan Wang,Yu Bai,Jayvon Nougaisse,Neal L Weintraub,Ming Guo,Xin-Yun Lu

Frontiers in oncology 11:740410 PubMed34926255

2021

Reinvestigating Tumor-Ventricle Relationship of Craniopharyngiomas With Predominantly Ventricular Involvement: An Endoscopic Endonasal Series Based on Histopathological Assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Fan,Yi Liu,Chaohu Wang,Zhanpeng Feng,Jun Pan,Yuping Peng,Junxiang Peng,Yun Bao,Jing Nie,Binghui Qiu,Songtao Qi
View all publications
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