Rabbit Recombinant Monoclonal Oxytocin-neurophysin 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 10 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-frozen-sections-img128180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135775.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)")
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-frozen-sections-img99650.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)")
![Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--western-blot-img148728.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107413.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (AB212193)")
Publications
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- Journal of virology 97:e01006232023Zika virus infection leads to hormone deficiencies of the hypothalamic-pituitary-gonadal axis and diminished fertility in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Bo Liu,Wei Yang,Jia-Tong Chang,Dong-Ying Fan,Yan-Hua Wu,Pei-Gang Wang,Jing AnPubMed 37732785
- Molecular psychiatry 27:4948-49582022Leptin enhances social motivation and reverses chronic unpredictable stress-induced social anhedonia during adolescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Lei,Dan Wang,Yu Bai,Jayvon Nougaisse,Neal L Weintraub,Ming Guo,Xin-Yun LuPubMed 36138127
- Frontiers in oncology 11:7404102021Reinvestigating Tumor-Ventricle Relationship of Craniopharyngiomas With Predominantly Ventricular Involvement: An Endoscopic Endonasal Series Based on Histopathological Assessment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Fan,Yi Liu,Chaohu Wang,Zhanpeng Feng,Jun Pan,Yuping Peng,Junxiang Peng,Yun Bao,Jing Nie,Binghui Qiu,Songtao QiPubMed 34926255
- Molecular autism 12:412021Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNick Sarn,Stetson Thacker,Hyunpil Lee,Charis EngPubMed 34088332
- Frontiers in cell and developmental biology 9:5957442021A Highly Conserved Enhancer Coordinates Hypothalamic and Craniofacial Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoe Crane-Smith,Jeffrey Schoenebeck,Katy A Graham,Paul S Devenney,Lorraine Rose,Mark Ditzell,Eve Anderson,Joseph I Thomson,Natasha Klenin,Deborah M Kurrasch,Laura A Lettice,Robert E HillPubMed 33869166
- The Journal of physiology 598:2355-23702020Regular moderate exercise alleviates gastric oxidative damage in rats via the contribution of oxytocin receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSevil Arabacı Tamer,Selen Üçem,Berk Büke,Muhammed Güner,Alp Giray Karaküçük,Niyazi Yiğit,Serap Şirvancı,Özge Çevik,Feriha Ercan,Berrak Ç YeğenPubMed 32266969
- Journal of chemical neuroanatomy 106:1017942020Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Zhou et. al.PubMed 32315740
- Journal of chemical neuroanatomy 98:97-1032019Distribution of D and D receptor- immunoreactive neurons in the paraventricular nucleus of the hypothalamus in the rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuerui Ran et. al.PubMed 31018158
- Frontiers in neuroscience 13:1222019Effects of Intranasal Oxytocin on Pup Deprivation-Evoked Aberrant Maternal Behavior and Hypogalactia in Rat Dams and the Underlying Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Yu Liu et. al.PubMed 30863276
- Frontiers in neuroscience 12:8072018Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang-Shan Yuan et. al.PubMed 30455627
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
| Rat | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/8000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/8000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PubMed:18174156).
Additional Targets
Oxytocin-neurophysin 1
Alternative names
OT, OXT, Oxytocin-neurophysin 1, OT-NPI
Recommended products
Rabbit Recombinant Monoclonal Oxytocin-neurophysin 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20973
- Purification technique
- Affinity purification Protein A
- Specificity
Specific expression in pituitary tissue in WB and posterior lobe in IHC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oxytocin-neurophysin 1 also known as prepro-oxytocin-neurophysin I is a precursor protein with a molecular weight of approximately 20 kDa. This protein is expressed primarily in the hypothalamus and is involved in the synthesis and storage of the hormone oxytocin a peptide essential for various physiological processes. During synthesis the protein undergoes cleavage to separate oxytocin and neurophysin 1 a carrier protein that aids in the transport and stabilization of oxytocin in neurosecretory granules.
- Biological function summary
Oxytocin-neurophysin 1 plays an important role in the regulation of social behaviors reproductive functions and fluid homeostasis. This precursor is not part of a larger protein complex but neurophysin 1 serves as an essential carrier that binds and transports oxytocin to its target sites in the body. Oxytocin itself functions as a neuromodulator and hormone exerting effects on the uterine muscles and mammary glands promoting childbirth and lactation.
- Pathways
The biosynthesis and release of oxytocin-neurophysin 1 are closely associated with the hypothalamic neurosecretory pathways. These pathways involve the neurohypophysis where oxytocin is released into the bloodstream. Additionally oxytocin interacts with the G-protein-coupled receptor OXTR to elicit its physiological effects. V1a and V2 receptors alongside oxytocin play roles in various signaling pathways that affect metabolic and cardiovascular functions.
- Associated diseases and disorders
Dysregulation of oxytocin-neurophysin 1 has been linked to the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and autism spectrum disorders. In these conditions altered levels of oxytocin affect fluid balance and social behavior. Additionally changes in oxytocin levels often in tandem with altered vasopressin regulation associate with the development and exacerbation of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-frozen-sections-img128180.jpg "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)")
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135775.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-frozen-sections-img99650.jpg "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)")
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--western-blot-img148728.jpg "Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)")
Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Exposure times: Lane 1: 3 seconds; Lane 2: 1 second.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) at 1/1000 dilution
Lane 1: Mouse pituitary tissue lysate at 10 µg
Lane 2: Rat pituitary tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 13 kDa
Observed band size: 12 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-ab212193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107413.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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