Rabbit Recombinant Monoclonal Oxytocin-neurophysin 1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-frozen-sections-img128180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135775.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490)")
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-frozen-sections-img99650.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107413.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (AB226490)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PubMed:18174156).
Additional Targets
Oxytocin-neurophysin 1
Alternative names
OT, OXT, Oxytocin-neurophysin 1, OT-NPI
Recommended products
Rabbit Recombinant Monoclonal Oxytocin-neurophysin 1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20973
- Purification technique
- Affinity purification Protein A
- Specificity
Specific expression in pituitary tissue in WB and posterior lobe in IHC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab226490 is the carrier-free version of Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oxytocin-neurophysin 1 also known as prepro-oxytocin-neurophysin I is a precursor protein with a molecular weight of approximately 20 kDa. This protein is expressed primarily in the hypothalamus and is involved in the synthesis and storage of the hormone oxytocin a peptide essential for various physiological processes. During synthesis the protein undergoes cleavage to separate oxytocin and neurophysin 1 a carrier protein that aids in the transport and stabilization of oxytocin in neurosecretory granules.
- Biological function summary
Oxytocin-neurophysin 1 plays an important role in the regulation of social behaviors reproductive functions and fluid homeostasis. This precursor is not part of a larger protein complex but neurophysin 1 serves as an essential carrier that binds and transports oxytocin to its target sites in the body. Oxytocin itself functions as a neuromodulator and hormone exerting effects on the uterine muscles and mammary glands promoting childbirth and lactation.
- Pathways
The biosynthesis and release of oxytocin-neurophysin 1 are closely associated with the hypothalamic neurosecretory pathways. These pathways involve the neurohypophysis where oxytocin is released into the bloodstream. Additionally oxytocin interacts with the G-protein-coupled receptor OXTR to elicit its physiological effects. V1a and V2 receptors alongside oxytocin play roles in various signaling pathways that affect metabolic and cardiovascular functions.
- Associated diseases and disorders
Dysregulation of oxytocin-neurophysin 1 has been linked to the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and autism spectrum disorders. In these conditions altered levels of oxytocin affect fluid balance and social behavior. Additionally changes in oxytocin levels often in tandem with altered vasopressin regulation associate with the development and exacerbation of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-frozen-sections-img128180.jpg "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)")
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135775.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin-neurophysin 1 with Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-frozen-sections-img99650.jpg "Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)")
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490), expandable thumbnail](https://content.abcam.com/products/images/oxytocin-neurophysin-1-antibody-epr20973-bsa-and-azide-free-ab226490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107413.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] - BSA and Azide free (ab226490)
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin-neurophysin 1 with Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Oxytocin-neurophysin 1 antibody [EPR20973] ab212193).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com