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Rabbit Recombinant Monoclonal Oxytocin Receptor antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443), expandable thumbnail
  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443), expandable thumbnail
  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytIP
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.

Alternative names

Oxytocin receptor, OT-R, OXTR

Recommended products

Rabbit Recombinant Monoclonal Oxytocin Receptor antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24409-146
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The oxytocin receptor (OTR) also known as OXTR is a G-protein-coupled receptor that primarily binds the neuropeptide hormone oxytocin. It has a molecular mass of approximately 43 kDa. This receptor is expressed in diverse tissues including the brain uterus mammary glands and heart. It plays a significant role in signal transduction processes converting extracellular hormonal signals into specific intracellular effects that facilitate various physiological functions.

Biological function summary

The oxytocin receptor exhibits involvement in many reproductive and social behaviors. Despite not functioning as part of a larger complex OTR activation facilitates processes such as uterine contraction milk ejection and social bonding. Activation of this receptor influences neuronal circuits in the brain supporting behaviors linked to emotion and social memory which are fundamental to maternal behaviors and pair bonding.

Pathways

The oxytocin receptor participates in critical signaling cascades including the phosphatidylinositol-calcium second messenger system. Through this pathway the receptor activates protein kinase C and increases intracellular calcium levels. This pathway often involves interaction with other proteins such as vasopressin receptors which have overlapping functions in fluid balance and cardiovascular regulation.

Associated diseases and disorders

The oxytocin receptor has associations with conditions like autism spectrum disorder and postpartum depression. These disorders suggest that altered oxytocin receptor signaling may contribute to atypical social behavior and mood regulation. Additionally the receptor's interaction with proteins like the vasopressin receptor influences susceptibility to these neuropsychiatric conditions highlighting its role in neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443), expandable thumbnail

    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28382233).

    The blot of lane 1 was developed using a high sensitivity ECL substrate.

    All lanes: Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

    Lane 3: C2C12 differentiation for 7 days, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Observed band size: 55 kDa

    Exposure time: 3min

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443), expandable thumbnail

    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11467983).

    Negative control: skeletal muscle  (PMID: 11467983).

    All lanes: Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 43 kDa

    Observed band size: 55 kDa

    Exposure time: 3min

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443), expandable thumbnail

    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11467983).

    Negative control: skeletal muscle  (PMID: 11467983).

    All lanes: Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1: Rat uterus tissue lysate at 20 µg

    Lane 2: Rat skeletal muscle tissue lysate at 20 µg

    Lane 3: Rat testis tissue lysate at 20 µg

    Lane 4: Mouse uterus tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 6: Mouse testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Observed band size: 55 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1(rat embryonic fibroblast) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Rat-1 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in U-2 OS cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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