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AB300443

Anti-Oxytocin Receptor antibody [EPR24409-146]

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(1 Publication)

Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) is a rabbit monoclonal antibody detecting Oxytocin Receptor in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Oxytocin receptor, OT-R, OXTR

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in U-2 OS cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1(rat embryonic fibroblast) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Rat-1 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)
  • WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11467983).

Negative control : skeletal muscle (PMID : 11467983).

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)
  • WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28382233).

The blot of lane 1 was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 3:

C2C12 differentiation for 7 days, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)
  • WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (AB300443)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11467983).

Negative control : skeletal muscle (PMID : 11467983).

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

Lane 1:

Rat uterus tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Lane 4:

Mouse uterus tissue lysate at 20 µg

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24409-146

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR24409-146] also available for your convenience: ab300443, Carrier free - ab300444

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The oxytocin receptor (OTR) also known as OXTR is a G-protein-coupled receptor that primarily binds the neuropeptide hormone oxytocin. It has a molecular mass of approximately 43 kDa. This receptor is expressed in diverse tissues including the brain uterus mammary glands and heart. It plays a significant role in signal transduction processes converting extracellular hormonal signals into specific intracellular effects that facilitate various physiological functions.
Biological function summary

The oxytocin receptor exhibits involvement in many reproductive and social behaviors. Despite not functioning as part of a larger complex OTR activation facilitates processes such as uterine contraction milk ejection and social bonding. Activation of this receptor influences neuronal circuits in the brain supporting behaviors linked to emotion and social memory which are fundamental to maternal behaviors and pair bonding.

Pathways

The oxytocin receptor participates in critical signaling cascades including the phosphatidylinositol-calcium second messenger system. Through this pathway the receptor activates protein kinase C and increases intracellular calcium levels. This pathway often involves interaction with other proteins such as vasopressin receptors which have overlapping functions in fluid balance and cardiovascular regulation.

The oxytocin receptor has associations with conditions like autism spectrum disorder and postpartum depression. These disorders suggest that altered oxytocin receptor signaling may contribute to atypical social behavior and mood regulation. Additionally the receptor's interaction with proteins like the vasopressin receptor influences susceptibility to these neuropsychiatric conditions highlighting its role in neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
See full target information OXTR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36613886

2022

Mechanism of Oxytocin-Induced Contraction in Rat Gastric Circular Smooth Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Alqudah,Rima Abdul Razzaq,Mahmoud A Alfaqih,Othman Al-Shboul,Ahmed Al-Dwairi,Safa Taha
View all publications
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Product promise

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