AB19350

Anti-P cadherin antibody [6A9]

Name: Anti-P cadherin antibody [6A9] (ab19350) | Abcam
Brand: Abcam Limited
SKU: AB19350
Price: 455 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal CADH3 antibody. Suitable for ICC, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (AB19350)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (AB19350)

ab19350 staining P-Cadherin in A431 cells (top panel) and MCF7 (negative cell line - bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (AB19350)

ab19350 staining P-Cadherin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Carrier free

Anti-P cadherin antibody [6A9] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A9

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.

Biological function summary

P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.

Pathways

Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.

P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 55:e13305 PubMed35794842

2022

Inhibition of L-type voltage-gated calcium channel-mediated Ca influx suppresses the collective migration and invasion of ameloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shujin Li,Hyun-Yi Kim,Dong-Joon Lee,Sung-Ho Park,Keishi Otsu,Hidemitsu Harada,Young-Soo Jung,Han-Sung Jung

Artificial cells, nanomedicine, and biotechnology 46:S651-S660 PubMed30311795

2018

Core/shell multicellular spheroids on chitosan as in vitro 3D coculture tumor models.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Wen Tsai,Jyh-Horng Wang,Tai-Horng Young

Experimental and therapeutic medicine 16:1095-1102 PubMed30112052

2018

Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced-podocytes.

Applications

Species

Human

Xiaojun Cai,Lei Wang,Xuling Wang,Fengyan Hou

Biomaterials 33:5221-9 PubMed22541538

2012

Mimicking normal tissue architecture and perturbation in cancer with engineered micro-epidermis.

Applications

ICC/IF

Species

Human

Julien E Gautrot,Chunming Wang,Xin Liu,Stephen J Goldie,Britta Trappmann,Wilhelm T S Huck,Fiona M Watt

Oncotarget 3:299-313 PubMed22431556

2012

BRCA1/p220 loss triggers BRCA1-IRIS overexpression via mRNA stabilization in breast cancer cells.

Applications

Species

Unspecified reactive species

Yoshiko Shimizu,Nicole Mullins,Zannel Blanchard,Wael M Elshamy

The Journal of cell biology 136:919-34 PubMed9049256

1997

Cross-talk between adherens junctions and desmosomes depends on plakoglobin.

Applications

Species

Human

J E Lewis,J K Wahl,K M Sass,P J Jensen,K R Johnson,M J Wheelock

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P cadherin antibody [6A9]

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (AB19350)

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (AB19350)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise