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AB233769

Anti-P cadherin antibody [6A9] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal CADH3 antibody. Carrier free. Suitable for ICC, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (AB233769)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (AB233769)

ab19350 staining P-Cadherin in A431 cells (top panel) and MCF7 (negative cell line - bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab19350).

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (AB233769)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] - BSA and Azide free (AB233769)

ab19350 staining P-Cadherin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab19350).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A9

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

ICC, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab233769 is the carrier-free version of ab19350.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.
Biological function summary

P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.

Pathways

Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.

P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
See full target information CDH3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:2004-2016 PubMed35030978

2022

Human bone marrow mesenchymal stem cells-derived exosomes attenuated prostate cancer progression via the miR-99b-5p/IGF1R axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shichun Jiang,Haiyu Chen,Kai He,Juan Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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