AB242060

Anti-P cadherin antibody [EPR22426]

RabMAb
Recombinant
20ul selling size
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(6 Publications)

Rabbit Recombinant Monoclonal CADH3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CDHP, CDH3, Cadherin-3, Placental cadherin, P-cadherin

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] (AB242060)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling P cadherin with ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in BxPC-3 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] (AB242060)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling P cadherin with ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in ductal epithelium of human breast (PMID : 25424750, 2702654) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling P cadherin with ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in A431 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling P cadherin with ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human breast cancer (PMID : 28084338) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-P cadherin antibody [EPR22426] (AB242060)

Exposure time : Lanes 1 and 2 : 136 seconds; Lane 3 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 2702654, 15548699).

All lanes:

Western blot - Anti-P cadherin antibody [EPR22426] (ab242060) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

false

Carrier free

Anti-P cadherin antibody [EPR22426] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-P cadherin antibody [EPR22426]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-P cadherin antibody [EPR22426]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22426

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.

Biological function summary

P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.

Pathways

Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.

P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115096 PubMed39700008

2024

P-cadherin mechanoactivates tumor-mesothelium metabolic coupling to promote ovarian cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Sally Kit Yan To,Katie Sze Wai Fung,Kun Wang,Jiangwen Zhang,Alfonso Hing Wan Ngan,Susan Yung,Tak Mao Chan,Carmen Chak Lui Wong,Philip Pun Ching Ip,Ling Peng,Hong-Yan Guo,Chi Bun Chan,Alice Sze Tsai Wong

Journal of diabetes investigation 15:300-314 PubMed38149724

2023

Cordyceps cicadae polysaccharides attenuate diabetic nephropathy via the miR-30a-3p/TRIM16 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zheng,Qin Xu,Yiwen Wang,Yifei Zhong,Rong Zhu

Cell cycle (Georgetown, Tex.) 22:1865-1883 PubMed37592763

2023

SNTB1 regulates colorectal cancer cell proliferation and metastasis through YAP1 and the WNT/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Zheng Li,Juan Jiang,Yang Lei,Jingmao Xie,Yihui Liu,Bo Yi

Cell death & disease 14:158 PubMed36841807

2023

DAPL1 prevents epithelial-mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Ma,Shuxian Han,Youjia Liu,Yu Chen,Pingping Li,Xiaoyan Liu,Lifu Chang,Ying-Ao Chen,Feng Chen,Qiang Hou,Ling Hou

BMC medical genomics 15:148 PubMed35787690

2022

Construction and validation of a prognostic risk model for breast cancer based on protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Xujun Zhang,Qingyi Cao,Jianing Chen,Chenhong Lin,Tianxin Xiang,Ping Zeng

Oncology letters 21:494 PubMed33968210

2021

MicroRNA-665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhui Fang,Yangqiu Bai,Lida Zhang,Songze Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P cadherin antibody [EPR22426]

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] (AB242060)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] (AB242060)

Western blot - Anti-P cadherin antibody [EPR22426] (AB242060)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise