Rabbit Recombinant Monoclonal CADH3 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135868.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204)")
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunocytochemistry-immunofluorescence-img20633.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204)")
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunocytochemistry-immunofluorescence-img110441.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (AB245204)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Alternative names
Cadherin-3, Placental cadherin, P-cadherin, CDHP, CDH3
Recommended products
Rabbit Recombinant Monoclonal CADH3 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22426
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab245204 is the carrier-free version of Anti-P cadherin antibody [EPR22426] ab242060.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.
- Biological function summary
P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.
- Pathways
Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.
- Associated diseases and disorders
P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135868.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling P cadherin with Anti-P cadherin antibody [EPR22426] ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in ductal epithelium of human breast (PMID: 25424750, 2702654) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P cadherin antibody [EPR22426] ab242060).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunocytochemistry-immunofluorescence-img20633.jpg "Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)")
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling P cadherin with Anti-P cadherin antibody [EPR22426] ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in BxPC-3 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P cadherin antibody [EPR22426] ab242060).
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunocytochemistry-immunofluorescence-img110441.jpg "Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)")
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling P cadherin with Anti-P cadherin antibody [EPR22426] ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in A431 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P cadherin antibody [EPR22426] ab242060).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204), expandable thumbnail](https://content.abcam.com/products/images/p-cadherin-antibody-epr22426-bsa-and-azide-free-ab245204--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66527.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling P cadherin with Anti-P cadherin antibody [EPR22426] ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human breast cancer (PMID: 28084338) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P cadherin antibody [EPR22426] ab242060).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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