Rabbit Polyclonal P Glycoprotein antibody. Suitable for IP, WB and reacts with Human samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ABCB1 aa 1-50.
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- Cellular and molecular biology (Noisy-le-Grand, France) 69:254-2592023Study on the reversal of epileptic drug resistance by tetramethylpyrazine in combination with carbamazepine through modulation of P-Glycoprotein expression in rat brain microvessel endothelial cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Qian,Yuqing Deng,Na Zhong,Yuxuan Li,Junhua Wang,Jinhua Yan,Wei Liu,Jinglei Ye,Jingchao Liu,Guobei XiaoPubMed 38015511
- Drug metabolism and disposition: the biological fate of chemicals 51:1177-11872023Improvement of Protein Expression Profile in Three-Dimensional Renal Proximal Tubular Epithelial Cell Spheroids Selected Based on OAT1 Gene Expression: A Potential In Vitro Tool for Evaluating Human Renal Proximal Tubular Toxicity and Drug Disposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoki Ishiguro,Etsushi Takahashi,Hiroshi Arakawa,Asami Saito,Fumihiko Kitagawa,Masayuki Kondo,Gaku Morinaga,Masahito Takatani,Ryo Takahashi,Takashi Kudo,Shin-Ichi Mae,Moeno Kadoguchi,Daichi Higuchi,Yuya Nakazono,Ikumi Tamai,Kenji Osafune,Yoichi JimboPubMed 37385755
- Frontiers in oncology 12:9664042022P2X7 receptor isoform B is a key drug resistance mediator for neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa Fernandes Arnaud-Sampaio,Carolina Adriane Bento,Talita Glaser,Elena Adinolfi,Henning Ulrich,Claudiana LameuPubMed 36091161
- Histology and histopathology 38:647-6582022Circular RNA circLRCH3 promotes oxaliplatin resistance in gastric cancer through the modulation of the miR-383-5p/FGF7 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengcheng Xiang,Rong Li,Huizhu Qiu,Erdong Zuo,Yuan Zhang,Li Shan,Xu ChengPubMed 35920365
- Cell biochemistry and function 40:391-4022022In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMostafa Mostafazadeh,Houman Kahroba,Sanya Haiaty,Abbas P TazeKand,Nasser Samadi,Reza Rahbarghazi,Mohammad NouriPubMed 35474580
- Bioengineered 13:5126-51402022Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can ZouPubMed 35170372
- Cancer cell international 21:3362021Circ_0001721 enhances doxorubicin resistance and promotes tumorigenesis in osteosarcoma through miR-758/TCF4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuapeng Guan,Hao Xu,Jinshui Chen,Weishan Wu,Dongfeng Chen,Yungang Chen,Jianzhong SunPubMed 34215252
- PloS one 16:e02367702021The blood-brain barrier studied in vitro across species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaj Schneider Thomsen,Nanna Humle,Eva Hede,Torben Moos,Annette Burkhart,Louiza Bohn ThomsenPubMed 33711041
- Journal of Cancer 12:2041-20492021Nodal is involved in chemoresistance of renal cell carcinoma cells via regulation of ABCB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingwang Zhu,Dongwei Xue,Jia Liu,Fengming Dong,Yongzhi Li,Yili LiuPubMed 33754002
- Experimental & molecular medicine 53:91-1022021METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Pan,Xiaolv Hong,Sumei Li,Ping Meng,Feng XiaoPubMed 33420414
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ABCB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P08183
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).
Alternative names
CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1
Recommended products
Rabbit Polyclonal P Glycoprotein antibody. Suitable for IP, WB and reacts with Human samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Human ABCB1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ABCB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P08183
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.
- Biological function summary
P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.
- Pathways
P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.
- Associated diseases and disorders
P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-P Glycoprotein antibody (ab129450)
All lanes: Western blot - Anti-P Glycoprotein antibody (ab129450) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 10s
Immunoprecipitation - Anti-P Glycoprotein antibody (ab129450)
Detection of P Glycoprotein by Western Blot of Immunprecipitate.
Lane 1: ab129450 at 1µg/ml staining P Glycoprotein in HeLa whole cell lysate immunoprecipitated using ab129450 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-P Glycoprotein antibody (ab129450)
Predicted band size: 141 kDa
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