AB129450

Anti-P Glycoprotein antibody

Name: Anti-P Glycoprotein antibody (ab129450) | Abcam
Brand: Abcam Limited
SKU: AB129450
Price: 450 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(57 Publications)

Rabbit Polyclonal P Glycoprotein antibody. Suitable for IP, WB and reacts with Human samples. Cited in 57 publications. Immunogen corresponding to Synthetic Peptide within Human ABCB1 aa 1-50.

View Alternative Names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

2 Images

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Immunoprecipitation - Anti-P Glycoprotein antibody (AB129450)

Detection of P Glycoprotein by Western Blot of Immunprecipitate.
Lane 1 : ab129450 at 1µg/ml staining P Glycoprotein in HeLa whole cell lysate immunoprecipitated using ab129450 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-P Glycoprotein antibody (ab129450)

Predicted band size: 141 kDa

false

Unknown

Western blot - Anti-P Glycoprotein antibody (AB129450)

All lanes:

Western blot - Anti-P Glycoprotein antibody (ab129450) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 141 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ABCB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P08183

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.

Biological function summary

P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.

Pathways

P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.

P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translocates drugs and phospholipids across the membrane (PubMed : 2897240, PubMed : 35970996, PubMed : 8898203, PubMed : 9038218, PubMed : 35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed : 8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed : 2897240, PubMed : 35970996, PubMed : 9038218).

See full target information ABCB1

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:8735 PubMed40082588

2025

Preclinical evaluation of a novel antibody-drug conjugate OBI-992 for Cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Chang,Chun-Jung Lin,Shih-Ni Wen,Yi-Chen Wu,Cheng-Yen Wei,Jye-Yu Huang,Yu-Hsuan Tsao,Yu-Jung Chen,Wei-Chien Tang,Yuen-Chin Wu,Wei-Han Lee,Teng-Yi Huang,Tzer-Min Kuo,Wan-Fen Li,Ming-Tain Lai

International journal of molecular sciences 25: PubMed39063014

2024

The Role of PTEN in Chemoresistance Mediated by the HIF-1α/YY1 Axis in Pediatric Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Antonio-Andres,Mario Morales-Martinez,Elva Jimenez-Hernandez,Sara Huerta-Yepez

Journal of biomedical research 38:382-396 PubMed38817007

2024

Timosaponin AⅢ induces drug-metabolizing enzymes by activating constitutive androstane receptor (CAR) dephosphorylation of the EGFR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Zubair Hafiz,Jie Pan,Zhiwei Gao,Ying Huo,Haobin Wang,Wei Liu,Jian Yang

Cellular and molecular biology (Noisy-le-Grand, France) 69:254-259 PubMed38015511

2023

Study on the reversal of epileptic drug resistance by tetramethylpyrazine in combination with carbamazepine through modulation of P-Glycoprotein expression in rat brain microvessel endothelial cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Qian,Yuqing Deng,Na Zhong,Yuxuan Li,Junhua Wang,Jinhua Yan,Wei Liu,Jinglei Ye,Jingchao Liu,Guobei Xiao

Drug metabolism and disposition: the biological fate of chemicals 51:1177-1187 PubMed37385755

2023

Improvement of Protein Expression Profile in Three-Dimensional Renal Proximal Tubular Epithelial Cell Spheroids Selected Based on OAT1 Gene Expression: A Potential In Vitro Tool for Evaluating Human Renal Proximal Tubular Toxicity and Drug Disposition.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Ishiguro,Etsushi Takahashi,Hiroshi Arakawa,Asami Saito,Fumihiko Kitagawa,Masayuki Kondo,Gaku Morinaga,Masahito Takatani,Ryo Takahashi,Takashi Kudo,Shin-Ichi Mae,Moeno Kadoguchi,Daichi Higuchi,Yuya Nakazono,Ikumi Tamai,Kenji Osafune,Yoichi Jimbo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300115 PubMed37156751

2023

The c-Src/LIST Positive Feedback Loop Sustains Tumor Progression and Chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xianteng Wang,Bing Wang,Fang Li,Xingkai Li,Ting Guo,Yushun Gao,Dawei Wang,Weiren Huang

Frontiers in oncology 12:966404 PubMed36091161

2022

P2X7 receptor isoform B is a key drug resistance mediator for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Fernandes Arnaud-Sampaio,Carolina Adriane Bento,Talita Glaser,Elena Adinolfi,Henning Ulrich,Claudiana Lameu

Histology and histopathology 38:647-658 PubMed35920365

2022

Circular RNA circLRCH3 promotes oxaliplatin resistance in gastric cancer through the modulation of the miR-383-5p/FGF7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Xiang,Rong Li,Huizhu Qiu,Erdong Zuo,Yuan Zhang,Li Shan,Xu Cheng

Cell biochemistry and function 40:391-402 PubMed35474580

2022

In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa Mostafazadeh,Houman Kahroba,Sanya Haiaty,Abbas P TazeKand,Nasser Samadi,Reza Rahbarghazi,Mohammad Nouri

Bioengineered 13:5126-5140 PubMed35170372

2022

Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-P Glycoprotein antibody

Immunoprecipitation - Anti-P Glycoprotein antibody (AB129450)

Western blot - Anti-P Glycoprotein antibody (AB129450)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (57)

Product promise