Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free (AB271923)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170903).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free (AB271923)

Immunohistochemical staining of paraffin embedded human kidney with purified ab170903 at a working dilution of 1/600. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170903).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free (AB271923)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170903).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free (AB271923)

Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170903).

• WB

Lab

Western blot - Anti-P Glycoprotein antibody [EPR10363] - BSA and Azide free (AB271923)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : P glycoprotein knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab170903 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab170903 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. ab170903 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170903).

All lanes:

Western blot - Anti-P Glycoprotein antibody [EPR10363] (<a href='/en-us/products/primary-antibodies/p-glycoprotein-antibody-epr10363-ab170903'>ab170903</a>)

Predicted band size: 141 kDa

false