Name: Anti-P Glycoprotein antibody [EPR10364-57]
SKU: ab170904
Rating: 4 (11 reviews)

Anti-P Glycoprotein antibody [EPR10364-57] is a rabbit recombinant monoclonal antibody that is used to detect P Glycoprotein in IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Recombinant format for unrivaled batch-batch consistency
- Specificity confirmed with ABCB1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Cited in over 190 publicati

Images

Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	IHC-P
Human	Not recommended	Tested	Tested
Mouse	Not recommended	Tested	Expected
Rat	Not recommended	Tested	Expected

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes For optimal detection Abcam recommends not boiling the sample after lysis.
Species Rat	Dilution info 1/1000 - 1/5000	Notes For optimal detection Abcam recommends not boiling the sample after lysis.
Species Human	Dilution info 1/1000 - 1/5000	Notes For optimal detection Abcam recommends not boiling the sample after lysis.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1200	Notes For unpurified use at 1/20 - 1/100 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

See full target information ABCB1

Function

Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).

Alternative names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10364-57
Purification technique: Affinity purification Protein A
Specificity: P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150-300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) specifically detects P Glycoprotein (UniProt ID: P08183; Molecular weight: 141kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has been confirmed by testing in knockout samples.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has been cited over 192 times in peer reviewed journals and is trusted by the scientific community.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has 10 independent reviews from customers.

Related Products
Antibody clone EPR10364-57 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 (ab225231).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 μg)
Lane 3: HepG2 whole cell lysate (20 μg)
Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. ab170904 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively.
Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Predicted band size: 141 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 0.1 µg/mL
Lanes 1 and 3: C6 (Rat glial tumor glial cell) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: C6 (Rat glial tumor glial cell) whole cell lysates prepared using 1%SDS Hot lysis method at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking/Dilution buffer: 5% NFDM/TBST.
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: C6 (Rat glial tumor cell line) cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image -10 seconds; Right image - 1 minute.
We suggest not to boil the sample after lysis.
For 1% SDS Hot Lysates preparation protocol, please refer to the protocol section of the website and/or here.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lanes 1 and 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1% SDS hot lysis method at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking/Dilution buffer: 5% NFDM/TBST.
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HepG2 (human hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image - 3 seconds; Right image - 10 seconds.
We suggest not to boil the sample after lysis.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lanes 1 and 3: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate prepared using 1%SDS lysis method at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg
Lane 4: Human fetal brain tissue lysate at 10 µg
Secondary
Lanes 1 - 3: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Lane 4: Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Rat kidney tissue lysate
Lane 4: Rat spleen tissue lysate
Predicted band size: 141 kDa
Observed band size: 180 kDa
Exposure time: 2min
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse spleen tissue lysate
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Observed band size: 180 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.