Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) is a rabbit monoclonal antibody detecting P Glycoprotein in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 190 publications
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ICC/IF | WB | IHC-P | |
---|---|---|---|
Human | Not recommended | Tested | Tested |
Mouse | Not recommended | Tested | Expected |
Rat | Not recommended | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
Species Human | Dilution info 1/1000 - 1/5000 | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1200 | Notes For unpurified use at 1/20 - 1/100 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).
CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) is a rabbit monoclonal antibody detecting P Glycoprotein in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 190 publications
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150-300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. ab170904 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively.
Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
Predicted band size: 141 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 0.1 µg/mL
Lanes 1 and 3: C6 (Rat glial tumor glial cell) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: C6 (Rat glial tumor glial cell) whole cell lysates prepared using 1%SDS Hot lysis method at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: C6 (Rat glial tumor cell line) cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image -10 seconds; Right image - 1 minute.
We suggest not to boil the sample after lysis.
For 1% SDS Hot Lysates preparation protocol, please refer to the protocol section of the website and/or here.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lanes 1 and 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1% SDS hot lysis method at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution
Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HepG2 (human hepatocellular carcinoma) whole cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image - 3 seconds; Right image - 10 seconds.
We suggest not to boil the sample after lysis.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution
Lanes 1 and 3: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared using RIPA lysis method at 15 µg
Lanes 2 and 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate prepared using 1%SDS lysis method at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg
Lane 4: Human fetal brain tissue lysate at 10 µg
Lanes 1 - 3: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Lane 4: Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Rat kidney tissue lysate
Lane 4: Rat spleen tissue lysate
Predicted band size: 141 kDa
Observed band size: 180 kDa
Exposure time: 2min
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse spleen tissue lysate
All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Observed band size: 180 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
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