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Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) is a rabbit monoclonal antibody detecting P Glycoprotein in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 190 publications


Images

Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (AB170904), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Expected
Rat
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Human
Dilution info
1/1000 - 1/5000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Tested
Tested

Species
Human
Dilution info
1/1200
Notes

For unpurified use at 1/20 - 1/100

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).

Alternative names

Recommended products

Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) is a rabbit monoclonal antibody detecting P Glycoprotein in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 190 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10364-57
Purification technique
Affinity purification Protein A
Specificity

P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150-300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) specifically detects P Glycoprotein (UniProt ID: P08183; Molecular weight: 141kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has been confirmed by testing in knockout samples.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has been cited over 192 times in peer reviewed journals and is trusted by the scientific community.
Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) has 10 independent reviews from customers.

Related Products
Antibody clone EPR10364-57 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 (Alexa Fluor® 647 Anti-P Glycoprotein antibody [EPR10364-57] ab225231).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. ab170904 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Predicted band size: 141 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 0.1 µg/mL

    Lanes 1 and 3: C6 (Rat glial tumor glial cell) whole cell lysates prepared using RIPA lysis method at 15 µg

    Lanes 2 and 4: C6 (Rat glial tumor glial cell) whole cell lysates prepared using 1%SDS Hot lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.

    Negative control: PBS instead of the primary antibody (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: C6 (Rat glial tumor cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: Left image -10 seconds; Right image - 1 minute.

    We suggest not to boil the sample after lysis.

    For 1% SDS Hot Lysates preparation protocol, please refer to the protocol section of the website and/or here.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution

    Lanes 1 and 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared using RIPA lysis method at 15 µg

    Lanes 2 and 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1% SDS hot lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337) at 1/2000 dilution

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: HepG2 (human hepatocellular carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: Left image - 3 seconds; Right image - 10 seconds.

    We suggest not to boil the sample after lysis.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution

    Lanes 1 and 3: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared using RIPA lysis method at 15 µg

    Lanes 2 and 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate prepared using 1%SDS lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

    Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

    Lane 4: Human fetal brain tissue lysate at 10 µg

    Secondary

    Lanes 1 - 3: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Lane 4: Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 141 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate

    Lane 2: Rat heart tissue lysate

    Lane 3: Rat kidney tissue lysate

    Lane 4: Rat spleen tissue lysate

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

    Exposure time: 2min

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate

    Lane 2: Mouse heart tissue lysate

    Lane 3: Mouse kidney tissue lysate

    Lane 4: Mouse spleen tissue lysate

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

    Exposure time: 2min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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