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Rabbit Recombinant Monoclonal P Glycoprotein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

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Images

Western blot - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (AB216656), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (AB216656), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (AB216656), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (AB216656), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (AB216656), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Rat
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Rat
Dilution info
-
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Human
Dilution info
-
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

Function

Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).

Alternative names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

Recommended products

Rabbit Recombinant Monoclonal P Glycoprotein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10364-57
Purification technique
Affinity purification Protein A
Specificity

P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab216656 is the carrier-free version of Anti-P Glycoprotein antibody [EPR10364-57] ab170904.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.

Biological function summary

P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.

Pathways

P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.

Associated diseases and disorders

P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    This WB data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - Anti-P Glycoprotein antibody [EPR10364-57] ab170904 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-P Glycoprotein antibody [EPR10364-57] ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. Anti-P Glycoprotein antibody [EPR10364-57] ab170904 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (Anti-P Glycoprotein antibody [EPR10364-57] ab170904)

    Predicted band size: 141 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    Immunohistochemical staining of paraffin embedded human kidney with purified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (inset).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at 1:1200 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.

    Negative control: PBS instead of the primary antibody (inset).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    This IHC data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling P Glycoprotein with unpurified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified Anti-P Glycoprotein antibody [EPR10364-57] ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364-57] ab170904).

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Product protocols

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