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Rabbit Recombinant Monoclonal P Glycoprotein antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.


Images

Western blot - Anti-P Glycoprotein antibody [EPR10364] (AB168337), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (AB168337), expandable thumbnail
  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (AB168337), expandable thumbnail
  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (AB168337), expandable thumbnail
  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (AB168337), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Rat
Dilution info
1/2000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Species
Human
Dilution info
1/2000
Notes

For optimal detection Abcam recommends not boiling the sample after lysis.

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).

Alternative names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

Recommended products

Rabbit Recombinant Monoclonal P Glycoprotein antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10364
Purification technique
Affinity purification Protein A
Specificity

P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab168337 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.

Biological function summary

P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.

Pathways

P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.

Associated diseases and disorders

P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: P glycoprotein knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: HepG2 cell lysate (20 μg)


    Lanes 1 - 4: Merged signal (red and green). Green - ab168337 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab168337 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. ab168337 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Predicted band size: 141 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab168337 at 1/100 dilution (14 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (Anti-P Glycoprotein antibody [EPR10364-57] ab170904) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: C6 (Rat glial tumor cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/2000 dilution

    Lanes 1 - 2: Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (Anti-P Glycoprotein antibody [EPR10364-57] ab170904) at 1/2000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: HepG2 (human hepatocellular carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 141 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/1000 dilution

    Lane 1: HeLa cell lysates at 10 µg

    Lane 2: 293T cell lysates at 10 µg

    Lane 3: Human fetal brain lysates at 10 µg

    Predicted band size: 141 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] (ab168337)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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