Rabbit Recombinant Monoclonal P Glycoprotein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--western-blot-img57133.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116628.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128269.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136089.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (AB271921)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
Species Mouse | Dilution info - | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
Species Rat | Dilution info - | Notes For optimal detection Abcam recommends not boiling the sample after lysis. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218).
Alternative names
CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1
Recommended products
Rabbit Recombinant Monoclonal P Glycoprotein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10364
- Purification technique
- Affinity purification Protein A
- Specificity
P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab168337 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
ab271921 is the carrier-free version of Anti-P Glycoprotein antibody [EPR10364] ab168337.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.
- Biological function summary
P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.
- Pathways
P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.
- Associated diseases and disorders
P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--western-blot-img57133.jpg "Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)")
Western blot - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: P glycoprotein knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-P Glycoprotein antibody [EPR10364] ab168337 observed at 160 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-P Glycoprotein antibody [EPR10364] ab168337 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. Anti-P Glycoprotein antibody [EPR10364] ab168337 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364] ab168337).
All lanes: Western blot - Anti-P Glycoprotein antibody [EPR10364] (Anti-P Glycoprotein antibody [EPR10364] ab168337)
Predicted band size: 141 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116628.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified Anti-P Glycoprotein antibody [EPR10364] ab168337 at 1/100 dilution (14 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364] ab168337).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128269.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling P Glycoprotein with unpurified Anti-P Glycoprotein antibody [EPR10364] ab168337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364] ab168337).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921), expandable thumbnail](https://content.abcam.com/products/images/p-glycoprotein-antibody-epr10364-bsa-and-azide-free-ab271921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136089.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364] - BSA and Azide free (ab271921)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P Glycoprotein with unpurified Anti-P Glycoprotein antibody [EPR10364] ab168337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-P Glycoprotein antibody [EPR10364] ab168337).
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