AB202976

Anti-P Glycoprotein antibody - N-terminal

Name: Anti-P Glycoprotein antibody - N-terminal (ab202976) | Abcam
Brand: Abcam Limited
SKU: AB202976
Rating: 3 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal P Glycoprotein antibody. N-terminal. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Abcb1a aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

1 Images

Supplier Data

Immunocytochemistry - Anti-P Glycoprotein antibody - N-terminal (AB202976)

Immunocytochemistry analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling P Glycoprotein with ab202976 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with Triton X-100 at room temperature for 20 minutes. Normal goat serum was used as the blockng buffer at 37°C for 20 minutes. Primary incuabtion was for 90 minutes at 37°C followed by a FITC conjugated Goat Anti-Rabbit IgG secondary antibody at 37°C for 90 minutes (green). Nuclear DNA was labelled with DAPI (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Mouse Abcb1a aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P21447

Specificity

The immunogen for ab202976 is derived from Mouse ABCB1A and is 75% cross-reactive with Mouse ABCB1B.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/50 - 1/200", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.

Biological function summary

P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.

Pathways

P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.

P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translocates drugs and phospholipids across the membrane (PubMed : 2897240, PubMed : 35970996, PubMed : 8898203, PubMed : 9038218, PubMed : 35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed : 8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed : 2897240, PubMed : 35970996, PubMed : 9038218).

See full target information ABCB1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS applied materials & interfaces 17:2884-2898 PubMed39752231

2025

Bola-Amphiphilic Dendrimer Enhances Imatinib to Target Metastatic Ovarian Cancer via β-Catenin-HRP2 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Shi,Margarita Artemenko,Weiyu Yu,Ming Zhang,Canhui Yi,Peng Chen,Shuting Lin,Zhancun Bian,Baoping Lian,Fanzhen Meng,Jiaxuan Chen,Tom Roussel,Ying Li,Karen K L Chan,Philip P C Ip,Hung-Cheng Lai,Sally K Y To,Xiaoxuan Liu,Ling Peng,Alice S T Wong

Experimental hematology & oncology 12:77 PubMed37679762

2023

CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dakui Luo,Min Chen,Qingguo Li,Kangjunjie Wang,Kaihua Wang,Junqiang Li,Guoxiang Fu,Zezhi Shan,Qi Liu,Yufei Yang,Lei Liang,Yanlei Ma,Yi Qin,Jun Qin,Daming Gao,Xinxiang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P Glycoprotein antibody - N-terminal

Immunocytochemistry - Anti-P Glycoprotein antibody - N-terminal (AB202976)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise