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AB272384

Anti-P-Selectin antibody (CD62P) [EPR22850-206] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CD62P antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

1 Images
Sandwich ELISA - Anti-P-Selectin antibody (CD62P) [EPR22850-206] - BSA and Azide free (Capture) (AB272384)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-P-Selectin antibody (CD62P) [EPR22850-206] - BSA and Azide free (Capture) (AB272384)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272202).

  • Unconjugated

    Anti-P-Selectin (CD62P) antibody [EPR22850-206]

  • Carrier free

    Anti-CD62P antibody [EPR22850-206] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22850-206

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab272384 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human P-Selectin. The recommended pair for sandwich ELISA is:
Capture: ab272384, Human P-Selectin Capture Antibody (unconjugated)
Detector: ab272385, Human P-Selectin Detector Antibody (unconjugated)
The reference range value is 100 - 6400 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P-Selectin also known as CD62P or GMP-140 is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It has a molecular weight of approximately 140 kDa. In resting cells P-Selectin resides within alpha granules of platelets and Weibel-Palade bodies of endothelial cells. Upon activation it rapidly translocates to the cell surface playing an important role in mediating the initial interactions between endothelial cells leukocytes and platelets by binding to its ligand P-Selectin glycoprotein ligand-1 (PSGL-1).
Biological function summary

P-Selectin facilitates the recruitment of leukocytes to the site of injury or inflammation. It is a member of the selectin family of cell adhesion molecules which also includes E-Selectin and L-Selectin. P-Selectin interactions promote rolling and tethering of leukocytes on the endothelium a critical step for leukocyte extravasation into tissues. These processes are important for innate immune responses and the inflammation process.

Pathways

P-Selectin acts within the coagulation and inflammation pathways. In the coagulation pathway P-Selectin enhances platelet aggregation by interacting with fibrin and promoting thrombus formation. In the inflammation pathway it interacts with chemokines and integrins such as ICAM-1 facilitating leukocyte adhesion and transmigration. By partnering with these molecules P-Selectin coordinates cellular events that lead to immune response and wound healing.

P-Selectin is implicated in atherosclerosis and thrombosis. In atherosclerosis excessive expression of P-Selectin contributes to the recruitment of monocytes to the arterial wall promoting foam cell formation and plaque growth. In thrombosis elevated P-Selectin levels enhance platelet-leukocyte interactions exacerbating thrombus formation. The protein VWF (von Willebrand factor) is closely related to P-Selectin in these conditions as it also promotes platelet adhesion and aggregation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (PubMed : 37184585).
See full target information SELP
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Product promise

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