AB244504

Anti-p107 antibody

Name: Anti-p107 antibody (ab244504) | Abcam
Brand: Abcam Limited
SKU: AB244504
Price: 630 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal p107 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBL1 aa 550 to C-terminus.

View Alternative Names

Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, pRb1, p107, RBL1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human testis tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human colon tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human kidney tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma cells stained for p107 (green) using ab244504 at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-p107 antibody (AB244504)

All lanes:

Western blot - Anti-p107 antibody (ab244504) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RBL1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P28749

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "Block in 100pc LICOR. Blocking in Milk does not work.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p107 also known as Retinoblastoma-like protein 1 (RBL1) is a member of the retinoblastoma (RB) protein family and has a molecular weight of approximately 107 kDa. p107 is known for its role as a cell cycle regulator specifically in controlling cell cycle progression at the G1 phase. It is widely expressed in many tissues including the brain skeletal muscle and lymphoid tissues. Like other members of the RB family p107 contains an RB pocket domain important for its interactions with other proteins.

Biological function summary

Retinoblastoma-like protein 1 functions in the regulation of the cell cycle acting as an important checkpoint controller between the G1 and S phases. This protein forms a complex with E2F transcription factors which are important in controlling genes involved in cell cycle progression. The p107-E2F interaction represses transcription and inhibits the cell from proceeding to the S phase thereby ensuring proper cellular proliferation control. Moreover p107 can bind to cyclin-dependent kinases adding another layer of regulation.

Pathways

P107 plays essential roles in the RB-E2F pathway and the DNA damage response pathway. Within these pathways p107 interacts with other key proteins like p130 and pRB which together form the pocket protein family. The RB-E2F pathway controls cell cycle entry and exit while the DNA damage response pathway ensures genomic integrity. p107 acts as a mediator to pause the cell cycle during DNA damage repair collaborating with these related proteins to maintain cellular homeostasis.

P107 shows notable implications in cancer and retinoblastoma. Alterations in the expression or function of p107 can lead to unregulated cell proliferation contributing to oncogenesis. In cancer disruptions in the RB-E2F pathway often involving mutations or changes in p107 pRB or related proteins can induce tumor formation. In retinoblastoma although pRB plays a more dominant role reduced functionality of p107 can exacerbate the disease by weakening cell cycle control. Therefore p107 occupies an essential position in both safeguarding against diseases and being a potential factor in disease progression when dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of entry into cell division (PubMed : 17671431). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity). Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity). Controls histone H4 'Lys-20' trimethylation (By similarity). Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation (PubMed : 8319904). May act as a tumor suppressor (PubMed : 8319904).

See full target information RBL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37580082

2023

Neuroprotective effects of hepatoma-derived growth factor in models of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Voelkl,Sara Gutiérrez-Ángel,Sophie Keeling,Seda Koyuncu,Miguel da Silva Padilha,Dennis Feigenbutz,Thomas Arzberger,David Vilchez,Rüdiger Klein,Irina Dudanova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p107 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

Western blot - Anti-p107 antibody (AB244504)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise