Rabbit Polyclonal p107 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RBL1 aa 1000 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RBL1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P28749
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 8 - 15 μl/mg |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Key regulator of entry into cell division (PubMed:17671431). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity). Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity). Controls histone H4 'Lys-20' trimethylation (By similarity). Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation (PubMed:8319904). May act as a tumor suppressor (PubMed:8319904).
Alternative names
Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, pRb1, p107, RBL1
Recommended products
Rabbit Polyclonal p107 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RBL1 aa 1000 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RBL1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P28749
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245514 was affinity purified using an epitope specific to p107 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab245514 has not performed satisfactorily when used for WB of p107 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of p107.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p107 also known as Retinoblastoma-like protein 1 (RBL1) is a member of the retinoblastoma (RB) protein family and has a molecular weight of approximately 107 kDa. p107 is known for its role as a cell cycle regulator specifically in controlling cell cycle progression at the G1 phase. It is widely expressed in many tissues including the brain skeletal muscle and lymphoid tissues. Like other members of the RB family p107 contains an RB pocket domain important for its interactions with other proteins.
- Biological function summary
Retinoblastoma-like protein 1 functions in the regulation of the cell cycle acting as an important checkpoint controller between the G1 and S phases. This protein forms a complex with E2F transcription factors which are important in controlling genes involved in cell cycle progression. The p107-E2F interaction represses transcription and inhibits the cell from proceeding to the S phase thereby ensuring proper cellular proliferation control. Moreover p107 can bind to cyclin-dependent kinases adding another layer of regulation.
- Pathways
P107 plays essential roles in the RB-E2F pathway and the DNA damage response pathway. Within these pathways p107 interacts with other key proteins like p130 and pRB which together form the pocket protein family. The RB-E2F pathway controls cell cycle entry and exit while the DNA damage response pathway ensures genomic integrity. p107 acts as a mediator to pause the cell cycle during DNA damage repair collaborating with these related proteins to maintain cellular homeostasis.
- Associated diseases and disorders
P107 shows notable implications in cancer and retinoblastoma. Alterations in the expression or function of p107 can lead to unregulated cell proliferation contributing to oncogenesis. In cancer disruptions in the RB-E2F pathway often involving mutations or changes in p107 pRB or related proteins can induce tumor formation. In retinoblastoma although pRB plays a more dominant role reduced functionality of p107 can exacerbate the disease by weakening cell cycle control. Therefore p107 occupies an essential position in both safeguarding against diseases and being a potential factor in disease progression when dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunoprecipitation - Anti-p107 antibody (ab245514)
p107 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab245514 at 8 μl per reaction. Western blot was performed on the immunoprecipitate using ab245514 at 1/400 dilution.
Lane 1: ab245514 IP in Jurkat cell lysate.
Lane 2: Control IgG IP in Jurkat cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-p107 antibody (ab245514)
Predicted band size: 121 kDa
Western blot - Anti-p107 antibody (ab245514)
False colour image of Western blot: Anti-p107 antibody staining at 1/400 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245514 was shown to bind specifically to p107. A band was observed at 121 kDa in wild-type HeLa cell lysates with no signal observed at this size in RBL1 knockout cell line Human RBL1 (p107) knockout HeLa cell line ab264706 (knockout cell lysate Human RBL1 (p107) knockout HeLa cell lysate ab258630). To generate this image, wild-type and RBL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-p107 antibody (ab245514) at 1/400 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RBL1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human RBL1 (p107) knockout HeLa cell line (Human RBL1 (p107) knockout HeLa cell line ab264706)
Lane 3: HCT 116 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 121 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com