Anti-p107 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-p107 antibody (AB245514)

p107 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab245514 at 8 μl per reaction. Western blot was performed on the immunoprecipitate using ab245514 at 1/400 dilution.

Lane 1 : ab245514 IP in Jurkat cell lysate.
Lane 2 : Control IgG IP in Jurkat cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-p107 antibody (ab245514)

Predicted band size: 121 kDa

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• WB

Lab

Western blot - Anti-p107 antibody (AB245514)

False colour image of Western blot : Anti-p107 antibody staining at 1/400 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245514 was shown to bind specifically to p107. A band was observed at 121 kDa in wild-type HeLa cell lysates with no signal observed at this size in RBL1 knockout cell line ab264706 (knockout cell lysate ab258630). To generate this image, wild-type and RBL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-p107 antibody (ab245514) at 1/400 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RBL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RBL1 (p107) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbl1-p107-knockout-hela-cell-line-ab264706'>ab264706</a>)

Lane 3:

HCT 116 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Predicted band size: 121 kDa

Observed band size: 121 kDa

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