AB96520

Anti-p114RhoGEF antibody

Name: Anti-p114RhoGEF antibody (ab96520) | Abcam
Brand: Abcam Limited
SKU: AB96520
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal p114RhoGEF antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF18 aa 600-850.

View Alternative Names

KIAA0521, ARHGEF18, Rho guanine nucleotide exchange factor 18, 114 kDa Rho-specific guanine nucleotide exchange factor, Septin-associated RhoGEF, p114-Rho-GEF, p114RhoGEF, SA-RhoGEF

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody (AB96520)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody (AB96520)

Immunohistochemical analysis of paraffin embedded mouse thymus gland tissue stained for ARHGEF18 protein at cytoplasm with ab96520 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody (AB96520)

Immunohistochemical analysis of paraffin embedded rat spleen tissue stained for ARHGEF18 protein at cell membrane and cytoplasm with ab96520 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-p114RhoGEF antibody (AB96520)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-p114RhoGEF antibody (ab96520) at 1/1000 dilution

All lanes:

NT2D1 Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 131 kDa

false

Supplier Data

Western blot - Anti-p114RhoGEF antibody (AB96520)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-p114RhoGEF antibody (ab96520) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 131 kDa

false

Supplier Data

Western blot - Anti-p114RhoGEF antibody (AB96520)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-p114RhoGEF antibody (ab96520) at 1/1000 dilution

All lanes:

Neuro2a Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 131 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGEF18 aa 600-850. The exact immunogen used to generate this antibody is proprietary information.

Q6ZSZ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P114RhoGEF also known as ARHGEF18 is a guanine nucleotide exchange factor (GEF) specific for Rho GTPases. It functions by facilitating the exchange of GDP for GTP on RhoA a process important for activating RhoA. p114RhoGEF is approximately 114 kDa in mass. It is expressed in various tissues including vascular endothelial cells and neurons indicating a diverse range of functional roles across different systems.

Biological function summary

This protein plays an important role in the regulation of the actin cytoskeleton dynamics and cell migration. p114RhoGEF associates with other proteins to form complexes that regulate cell shape and motility. It binds with G-proteins and other signaling molecules influencing cellular responses to environmental cues such as changes in cell adhesion or polarity.

Pathways

P114RhoGEF is involved in the Rho signaling pathway which is essential for maintaining cell structure and function. Within this pathway it interacts closely with proteins like ZO-1 and Gα12/13 to control stress fiber formation and focal adhesion. It also features in pathways regulating epithelial cell junctions and endothelial barrier integrity which are critical for tissue homeostasis and vascular function.

P114RhoGEF plays a notable role in cancer progression and cardiovascular diseases. Disruptions in its function can lead to aberrant cell migration and invasion factors linked to tumor metastasis. Additionally changes in its activity might influence endothelial barrier function contributing to vascular leakiness associated with inflammatory conditions. Related proteins such as RhoA and ZO-1 also partake in these pathological processes highlighting the significance of p114RhoGEF in health and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed : 22006950).

See full target information ARHGEF18

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed34368835

2021

Enhanced RhoA signalling stabilizes E-cadherin in migrating epithelial monolayers.

Applications

Unspecified application

Species

Unspecified reactive species

Shafali Gupta,Kinga Duszyc,Suzie Verma,Srikanth Budnar,Xuan Liang,Guillermo A Gomez,Philippe Marcq,Ivar Noordstra,Alpha S Yap

Cell reports 32:107924 PubMed32697990

2020

Interplay between Extracellular Matrix Stiffness and JAM-A Regulates Mechanical Load on ZO-1 and Tight Junction Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Alexis J Haas,Ceniz Zihni,Artur Ruppel,Christian Hartmann,Klaus Ebnet,Masazumi Tada,Maria S Balda,Karl Matter

Developmental cell 47:439-452.e6 PubMed30318244

2018

A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Alexander Nestor-Bergmann,Xuan Liang,Shafali Gupta,Kinga Duszyc,Estelle Gauquelin,Guillermo A Gomez,Srikanth Budnar,Philippe Marcq,Oliver E Jensen,Zev Bryant,Alpha S Yap

PloS one 8:e68291 PubMed23826386

2013

MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1.

Applications

Species

Human

Jianran Hu,Yu Li,Ping Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p114RhoGEF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody (AB96520)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody (AB96520)

Western blot - Anti-p114RhoGEF antibody (AB96520)

Western blot - Anti-p114RhoGEF antibody (AB96520)

Western blot - Anti-p114RhoGEF antibody (AB96520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise