Rabbit Polyclonal p115-RhoGEF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF1 aa 200-350.
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- Nature communications 13:21792022Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian GuoPubMed 35449133
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGEF1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q92888
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed:9641915, PubMed:9641916). Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase (PubMed:9641915, PubMed:9641916, PubMed:8810315, PubMed:30521495). Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain (PubMed:9641916). This GEF activity is inhibited by binding to activated GNA12 (PubMed:9641916). Mediates angiotensin-2-induced RhoA activation (PubMed:20098430).
Alternative names
Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, Sub1.5, p115-RhoGEF, p115RhoGEF, ARHGEF1
Recommended products
Rabbit Polyclonal p115-RhoGEF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF1 aa 200-350.
Alternative names
Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, Sub1.5, p115-RhoGEF, p115RhoGEF, ARHGEF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGEF1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q92888
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p115-RhoGEF also known as ARHGEF1 is a guanine nucleotide exchange factor that primarily activates RhoA a small GTPase. It has a molecular mass of approximately 115 kDa. You find p115-RhoGEF predominantly expressed in the heart lung and brain tissues. This protein facilitates the exchange of GDP for GTP on RhoA triggering downstream signaling events essential for cellular functions like growth motility and organization of cytoskeletal structures.
- Biological function summary
P115-RhoGEF plays an important role in regulating actin cytoskeleton dynamics and transmitting signals from G-protein-coupled receptors. The protein is part of a signaling complex that includes heterotrimeric G proteins. Through its influence on the actin cytoskeleton p115-RhoGEF helps control changes in cell shape and movement. These activities contribute significantly to various cellular processes including differentiation and migration.
- Pathways
P115-RhoGEF is an essential component in the RhoA signaling pathway. This pathway regulates cytoskeletal dynamics and has critical implications for processes like cell adhesion and migration. p115-RhoGEF collaborates with other proteins such as Gα12/13 subunits to mediate its signaling effects. Besides the RhoA pathway p115-RhoGEF also interacts with the PI3K/Akt pathway influencing cellular survival and apoptosis.
- Associated diseases and disorders
Disruptions in p115-RhoGEF activity link to cardiovascular diseases and certain forms of cancer. Alterations in its function may lead to improper cell movement contributing to metastasis in cancer development. Additionally abnormal regulation of RhoA signaling connected to p115-RhoGEF could compromise vascular integrity implicating this protein in hypertension. During disease progression p115-RhoGEF interacts with other proteins like ROCK1 to exert these pathological effects.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
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7 product images
Western blot - Anti-p115-RhoGEF antibody (ab223759)
False colour image of Western blot: Anti-p115-RhoGEF antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223759 was shown to bind specifically to p115-RhoGEF. A band was observed at 85 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Arhgef1 knockout cell line Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell line ab266667 (knockout cell lysate Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell lysate ab257842). To generate this image, wild-type and Arhgef1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-p115-RhoGEF antibody (ab223759) at 0.04 µg/mL
Lane 1: Wild-type HEK-293T Scraped cell lysate at 20 µg
Lane 2: ARHGEF1 knockout HEK-293T Scraped cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)
Paraffin embedded sections of human small intestine tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody (ab223759)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p115-RhoGEF (green) using ab223759 at 4 μg/ml in ICC.
Western blot - Anti-p115-RhoGEF antibody (ab223759)
All lanes: Western blot - Anti-p115-RhoGEF antibody (ab223759) at 0.4 µg/mL
Lane 1: U-937 MG
Lane 2: SCLC-21H
Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)
Paraffin embedded sections of human lymph node tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)
Paraffin embedded sections of human lung tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)
Paraffin embedded sections of human liver tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis. No positivity observed.
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