Anti-p115-RhoGEF antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody (AB223759)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p115-RhoGEF (green) using ab223759 at 4 μg/ml in ICC.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (AB223759)

Paraffin embedded sections of human small intestine tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (AB223759)

Paraffin embedded sections of human lymph node tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (AB223759)

Paraffin embedded sections of human lung tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (AB223759)

Paraffin embedded sections of human liver tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis. No positivity observed.

• WB

Lab

Western blot - Anti-p115-RhoGEF antibody (AB223759)

False colour image of Western blot : Anti-p115-RhoGEF antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223759 was shown to bind specifically to p115-RhoGEF. A band was observed at 85 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Arhgef1 knockout cell line ab266667 (knockout cell lysate ab257842). To generate this image, wild-type and Arhgef1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-p115-RhoGEF antibody (ab223759) at 0.04 µg/mL

Lane 1:

Wild-type HEK-293T Scraped cell lysate at 20 µg

Lane 2:

ARHGEF1 knockout HEK-293T Scraped cell lysate at 20 µg

Lane 2:

Western blot - Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-arhgef1-p115-rhogef-knockout-hek-293t-cell-line-ab266667'>ab266667</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

U-2 OS cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 85 kDa

false

• WB

Supplier Data

Western blot - Anti-p115-RhoGEF antibody (AB223759)

All lanes:

Western blot - Anti-p115-RhoGEF antibody (ab223759) at 0.4 µg/mL

Lane 1:

U-937 MG

Lane 2:

SCLC-21H

Predicted band size: 102 kDa

false