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AB255755

Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free

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Armenian hamster Recombinant Monoclonal p115-RhoGEF antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

View Alternative Names

Lbcl2, Lsc, Arhgef1, Rho guanine nucleotide exchange factor 1, Lbc's second cousin, Lymphoid blast crisis-like 2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A20 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

Flow Cytometry (Intracellular) - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL-4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/40 dilution (1μg) (Red) compared with an Armenian hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab243248.

Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL4 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

Western blot - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)
  • WB

Lab

Western blot - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (AB255755)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11384980).

Lysates should be made freshly and used in WB immediately.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1/2：3 seconds; Lane 3：15 seconds; Lane 4：37 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

All lanes:

Western blot - Anti-p115-RhoGEF antibody [JH-1] (<a href='/en-us/products/primary-antibodies/p115-rhogef-antibody-jh-1-ab243248'>ab243248</a>) at 1/1000 dilution

Lane 1:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 10 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-armenian-hamster-igg-h-l-hrp-ab5745'>ab5745</a>) at 1/5000 dilution

Predicted band size: 102 kDa

Observed band size: 115 kDa

false

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

JH-1

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab255755 is the carrier-free version of ab243248.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p115-RhoGEF also known as ARHGEF1 is a guanine nucleotide exchange factor that primarily activates RhoA a small GTPase. It has a molecular mass of approximately 115 kDa. You find p115-RhoGEF predominantly expressed in the heart lung and brain tissues. This protein facilitates the exchange of GDP for GTP on RhoA triggering downstream signaling events essential for cellular functions like growth motility and organization of cytoskeletal structures.
Biological function summary

P115-RhoGEF plays an important role in regulating actin cytoskeleton dynamics and transmitting signals from G-protein-coupled receptors. The protein is part of a signaling complex that includes heterotrimeric G proteins. Through its influence on the actin cytoskeleton p115-RhoGEF helps control changes in cell shape and movement. These activities contribute significantly to various cellular processes including differentiation and migration.

Pathways

P115-RhoGEF is an essential component in the RhoA signaling pathway. This pathway regulates cytoskeletal dynamics and has critical implications for processes like cell adhesion and migration. p115-RhoGEF collaborates with other proteins such as Gα12/13 subunits to mediate its signaling effects. Besides the RhoA pathway p115-RhoGEF also interacts with the PI3K/Akt pathway influencing cellular survival and apoptosis.

Disruptions in p115-RhoGEF activity link to cardiovascular diseases and certain forms of cancer. Alterations in its function may lead to improper cell movement contributing to metastasis in cancer development. Additionally abnormal regulation of RhoA signaling connected to p115-RhoGEF could compromise vascular integrity implicating this protein in hypertension. During disease progression p115-RhoGEF interacts with other proteins like ROCK1 to exert these pathological effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.
See full target information Arhgef1
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