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AB235275

Anti-p130 antibody

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Rabbit Polyclonal p130 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

1 Images
Western blot - Anti-p130 antibody (AB235275)
  • WB

Supplier Data

Western blot - Anti-p130 antibody (AB235275)

Human Protein Atlas : Cell line RNA database 22.9 NX = MOLT4.

IP experiment was performed but did not successfully pull-down p130 in MOLT4 lysate. Antibody is not recommended for IP under these conditions.

All lanes:

Western blot - Anti-p130 antibody (ab235275) at 1/1000 dilution

Lane 1:

MOLT4 whole cell lysate at 1 µg

Lane 2:

MOLT4 whole cell lysate at 10 µg

Lane 3:

Human eye tissue at 1 µg

Lane 4:

Human eye tissue at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG peroxidase conjugated at 1/10000 dilution

Predicted band size: 128 kDa

Observed band size: 125 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Recommended for human samples only.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P130 also known as RBL2 (retinoblastoma-like 2) is a member of the retinoblastoma (Rb) protein family which includes pRb and p107. This protein has a molecular weight of about 130 kDa. It plays roles in regulating cell cycle by binding to E2F transcription factors consequently suppressing cell-cycle progression from G1 to S phase. p130 is ubiquitously expressed in various tissues but it shows higher levels in tissues with a low proliferation rate.
Biological function summary

The retinoblastoma-like 2 (p130) functions as an important regulator within the cell cycle checkpoint machinery. p130 forms a complex with E2F transcription factors selectively inhibiting their transcriptional activity. This inhibition plays an integral role in controlling cellular proliferation and ensuring that DNA replication does not occur unchecked. It also associates with cyclin-dependent kinase inhibitors further influencing the progression of the cell cycle.

Pathways

P130 is implicated in the control of the G1/S transition within the cell cycle pathway. Its function is closely linked with the Rb/E2F pathway which is critical for the regulation of cell proliferation. p130 collaborates with Rb and p107 within this pathway ensuring proper control of cell cycle transitions and maintenance of cellular growth.

Retinoblastoma-like 2 (p130) shows significant connections to cancer and neurodegenerative disorders. Dysregulation of p130 along with its pathway partners like Rb can lead to unregulated cell growth contributing to oncogenesis. Additionally alterations in p130 function have implications in the development of Alzheimer's disease where cell cycle dysregulation occurs. Understanding p130's roles more deeply could provide insight into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
See full target information RBL2
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Product promise

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