AB76255

Anti-p130 (phospho S672) antibody [EP2126Y]

RabMAb
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(11 Publications)

Rabbit Monoclonal p130 phospho S672 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

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Lab

Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

Purified ab76255 at 1/20 dilution (1μg) immunoprecipitating p130 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.

Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate 10μg

Lane 2 (+) : ab76255 + Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76255 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.

Blocking Buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM/TBST.

Observed band size : 90-130 kDa

All lanes:

Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)

Predicted band size: 128 kDa

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Supplier Data

Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

Control : Rabbit monoclonal [EP2141Y] to p130 (ab76234)

All lanes:

Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution

Lane 1:

Jurkat cell lysate, treated with AP at 10 µg

Lane 2:

Jurkat cell lysate, untreated at 10 µg

Secondary

All lanes:

Goat Anti-rabbit (HRP) at 1/2000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Carrier free

Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2126Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P130 also known as RBL2 (retinoblastoma-like 2) is a member of the retinoblastoma (Rb) protein family which includes pRb and p107. This protein has a molecular weight of about 130 kDa. It plays roles in regulating cell cycle by binding to E2F transcription factors consequently suppressing cell-cycle progression from G1 to S phase. p130 is ubiquitously expressed in various tissues but it shows higher levels in tissues with a low proliferation rate.

Biological function summary

The retinoblastoma-like 2 (p130) functions as an important regulator within the cell cycle checkpoint machinery. p130 forms a complex with E2F transcription factors selectively inhibiting their transcriptional activity. This inhibition plays an integral role in controlling cellular proliferation and ensuring that DNA replication does not occur unchecked. It also associates with cyclin-dependent kinase inhibitors further influencing the progression of the cell cycle.

Pathways

P130 is implicated in the control of the G1/S transition within the cell cycle pathway. Its function is closely linked with the Rb/E2F pathway which is critical for the regulation of cell proliferation. p130 collaborates with Rb and p107 within this pathway ensuring proper control of cell cycle transitions and maintenance of cellular growth.

Retinoblastoma-like 2 (p130) shows significant connections to cancer and neurodegenerative disorders. Dysregulation of p130 along with its pathway partners like Rb can lead to unregulated cell growth contributing to oncogenesis. Additionally alterations in p130 function have implications in the development of Alzheimer's disease where cell cycle dysregulation occurs. Understanding p130's roles more deeply could provide insight into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

See full target information RBL2 pS672

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1615300 PubMed40703674

2025

inhibits porcine epidemic diarrhea virus infection by disrupting nucleocapsid protein-p53 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Hongde Lu,Haoyang Liu,Ning Guo,Yu Zhou,Haiyan Lu,Zhiyuan He,Hong Dong

Experimental and therapeutic medicine 26:330 PubMed37346406

2023

Activation of Wnt/β‑Catenin‑p130/E2F4 promotes the differentiation of bone marrow‑derived mesenchymal stem cells into type II alveolar epithelial cells through cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Xiwen Zhang,Ming Xue,Airan Liu,Haibo Qiu,Fengmei Guo

Cell reports 38:110448 PubMed35235778

2022

CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Erik S Knudsen,Vishnu Kumarasamy,Ram Nambiar,Joel D Pearson,Paris Vail,Hanna Rosenheck,Jianxin Wang,Kevin Eng,Rod Bremner,Daniel Schramek,Seth M Rubin,Alana L Welm,Agnieszka K Witkiewicz

eLife 10: PubMed34851822

2021

Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor P Enrico,Wayne Stallaert,Elizaveta T Wick,Peter Ngoi,Xianxi Wang,Seth M Rubin,Nicholas G Brown,Jeremy E Purvis,Michael J Emanuele

Journal of virology 95:e0018721 PubMed34037422

2021

Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjun Su,Da Shi,Xiaoxu Xing,Shanshan Qi,Dan Yang,Jiyu Zhang,Yuru Han,Qinghe Zhu,Haibo Sun,Xiaoran Wang,Haoyang Wu,Meijiao Wang,Shan Wei,Chunqiu Li,Donghua Guo,Li Feng,Dongbo Sun

Theranostics 11:3452-3471 PubMed33537097

2021

Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eva M Verdugo-Sivianes,Ana M Rojas,Sandra Muñoz-Galván,Daniel Otero-Albiol,Amancio Carnero

Breast cancer research : BCR 21:146 PubMed31852484

2019

Elacestrant (RAD1901) exhibits anti-tumor activity in multiple ER+ breast cancer models resistant to CDK4/6 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hitisha K Patel,Nianjun Tao,Kyung-Min Lee,Mariela Huerta,Heike Arlt,Tara Mullarkey,Steven Troy,Carlos L Arteaga,Teeru Bihani

Oncogene 38:4962-4976 PubMed30833638

2019

Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Schade,Matthew G Oser,Hilary E Nicholson,James A DeCaprio

Nucleic acids research 45:9960-9975 PubMed28973440

2017

MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Josué Álvaro-Blanco,Katia Urso,Yuri Chiodo,Carla Martín-Cortázar,Omar Kourani,Pablo Gómez-Del Arco,María Rodríguez-Martínez,Esther Calonge,José Alcamí,Juan Miguel Redondo,Teresa Iglesias,Miguel R Campanero

The Journal of biological chemistry 292:6583-6599 PubMed28289097

2017

Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Iwahori,Angie C Umaña,Halena R VanDeusen,Robert F Kalejta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p130 (phospho S672) antibody [EP2126Y]

Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise