Rabbit Monoclonal p130 phospho S672 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (AB284755), expandable thumbnail](https://content.abcam.com/products/images/p130-phospho-s672-antibody-ep2126y-bsa-and-azide-free-ab284755--western-blot-img20064.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (AB284755)")
![Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (AB284755), expandable thumbnail](https://content.abcam.com/products/images/p130-phospho-s672-antibody-ep2126y-bsa-and-azide-free-ab284755--immunoprecipitation-img9247.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (AB284755)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Alternative names
RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2
Recommended products
Rabbit Monoclonal p130 phospho S672 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2126Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284755 is the carrier-free version of Anti-p130 (phospho S672) antibody [EP2126Y] ab76255
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P130 also known as RBL2 (retinoblastoma-like 2) is a member of the retinoblastoma (Rb) protein family which includes pRb and p107. This protein has a molecular weight of about 130 kDa. It plays roles in regulating cell cycle by binding to E2F transcription factors consequently suppressing cell-cycle progression from G1 to S phase. p130 is ubiquitously expressed in various tissues but it shows higher levels in tissues with a low proliferation rate.
- Biological function summary
The retinoblastoma-like 2 (p130) functions as an important regulator within the cell cycle checkpoint machinery. p130 forms a complex with E2F transcription factors selectively inhibiting their transcriptional activity. This inhibition plays an integral role in controlling cellular proliferation and ensuring that DNA replication does not occur unchecked. It also associates with cyclin-dependent kinase inhibitors further influencing the progression of the cell cycle.
- Pathways
P130 is implicated in the control of the G1/S transition within the cell cycle pathway. Its function is closely linked with the Rb/E2F pathway which is critical for the regulation of cell proliferation. p130 collaborates with Rb and p107 within this pathway ensuring proper control of cell cycle transitions and maintenance of cellular growth.
- Associated diseases and disorders
Retinoblastoma-like 2 (p130) shows significant connections to cancer and neurodegenerative disorders. Dysregulation of p130 along with its pathway partners like Rb can lead to unregulated cell growth contributing to oncogenesis. Additionally alterations in p130 function have implications in the development of Alzheimer's disease where cell cycle dysregulation occurs. Understanding p130's roles more deeply could provide insight into therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755), expandable thumbnail](https://content.abcam.com/products/images/p130-phospho-s672-antibody-ep2126y-bsa-and-azide-free-ab284755--western-blot-img20064.jpg "Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755)")
Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755)
This data was developed using Anti-p130 (phospho S672) antibody [EP2126Y] ab76255, the same antibody clone in a different buffer formulation.
Control: Rabbit monoclonal [EP2141Y] to p130 (Anti-p130 antibody [EP2141Y] ab76234)
All lanes: Western blot - Anti-p130 (phospho S672) antibody [EP2126Y] (Anti-p130 (phospho S672) antibody [EP2126Y] ab76255) at 1/10000 dilution
Lane 1: Jurkat cell lysate, treated with AP at 10 µg
Lane 2: Jurkat cell lysate, untreated at 10 µg
SecondaryAll lanes: Goat Anti-rabbit (HRP) at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDa
![Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755), expandable thumbnail](https://content.abcam.com/products/images/p130-phospho-s672-antibody-ep2126y-bsa-and-azide-free-ab284755--immunoprecipitation-img9247.jpg "Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755)")
Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] - BSA and Azide free (ab284755)
This data was developed using Anti-p130 (phospho S672) antibody [EP2126Y] ab76255, the same antibody clone in a different buffer formulation.
Purified Anti-p130 (phospho S672) antibody [EP2126Y] ab76255 at 1/20 dilution (1μg) immunoprecipitating p130 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate 10μg
Lane 2 (+): Anti-p130 (phospho S672) antibody [EP2126Y] ab76255 + Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p130 (phospho S672) antibody [EP2126Y] ab76255 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90-130 kDa
All lanes: Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (Anti-p130 (phospho S672) antibody [EP2126Y] ab76255)
Predicted band size: 128 kDa
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