AB251534

Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal p130 phospho T986 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

5 Images

Flow Cytometry (Intracellular) - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : NIH/3T3 whole cell lysate 10μg (Input).
Lane 2 : ab211928 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] (<a href='/en-us/products/primary-antibodies/p130-phospho-t986-antibody-epr23892-ab211928'>ab211928</a>)

Predicted band size: 128 kDa

false

Supplier Data

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 2% BSA.

All lanes:

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (<a href='/en-us/products/primary-antibodies/p130-phospho-t986-antibody-epr23892-ab211928'>ab211928</a>) at 1/1000 dilution

Lane 1:

Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (<a href='/en-us/products/primary-antibodies/p130-phospho-t986-antibody-epr23892-ab211928'>ab211928</a>) at 1/1000 dilution

Lane 1:

Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg with single phospho peptide

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg with non phospho peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

This data was developed using ab211928, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (<a href='/en-us/products/primary-antibodies/p130-phospho-t986-antibody-epr23892-ab211928'>ab211928</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Exposure time: 30s

Unconjugated

Anti-p130 (phospho T986) antibody [EPR2389(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2389(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab251534 is the carrier-free version of ab211928.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P130 also known as RBL2 (retinoblastoma-like 2) is a member of the retinoblastoma (Rb) protein family which includes pRb and p107. This protein has a molecular weight of about 130 kDa. It plays roles in regulating cell cycle by binding to E2F transcription factors consequently suppressing cell-cycle progression from G1 to S phase. p130 is ubiquitously expressed in various tissues but it shows higher levels in tissues with a low proliferation rate.

Biological function summary

The retinoblastoma-like 2 (p130) functions as an important regulator within the cell cycle checkpoint machinery. p130 forms a complex with E2F transcription factors selectively inhibiting their transcriptional activity. This inhibition plays an integral role in controlling cellular proliferation and ensuring that DNA replication does not occur unchecked. It also associates with cyclin-dependent kinase inhibitors further influencing the progression of the cell cycle.

Pathways

P130 is implicated in the control of the G1/S transition within the cell cycle pathway. Its function is closely linked with the Rb/E2F pathway which is critical for the regulation of cell proliferation. p130 collaborates with Rb and p107 within this pathway ensuring proper control of cell cycle transitions and maintenance of cellular growth.

Retinoblastoma-like 2 (p130) shows significant connections to cancer and neurodegenerative disorders. Dysregulation of p130 along with its pathway partners like Rb can lead to unregulated cell growth contributing to oncogenesis. Additionally alterations in p130 function have implications in the development of Alzheimer's disease where cell cycle dysregulation occurs. Understanding p130's roles more deeply could provide insight into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

See full target information RBL2 pT986

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:545-552 PubMed31289526

2019

Lymphoepithelioma-like gastric carcinoma treated with partial gastrectomy: Two case reports.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Cao,Jun Xie,Yongxiang Qian,Yugang Wu,Zhaoqing Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] - BSA and Azide free (AB251534)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise